Structure of PDB 1r49 Chain A

Receptor sequence

>1r49A (length=548) Species: 9606 (Homo sapiens)

KWKWWEEERYPEGIKWKFLEHKGPVFAPPYEPLPENVKFYYDGKVMKLSP
KAEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNEEKNIITNLSKCD
FTQMSQYFKAQTEARKQMSKEEKLKIKEENEKLLKEYGFCIMDNHKERIA
NFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSDAKVPSPPPGHKWK
EVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKK
CVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTV
GCCSLRVEHINLHPELDGQEYVVEFDFLGRDSIRYYNKVPVEKRVFKNLQ
LFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQL
KELTAPDENIPAKILSYNRANRAVAILCNHQMMNLQTKIDAKKEQLADAR
RDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQLMKLEVQATDREENK
QSKLNFLDPRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMADEDYEF

3D structure

• PDB: 1r49 The role of lysine 532 in the catalytic mechanism of human topoisomerase I.
• Chain: A
• Resolution: 3.13 Å

Enzymatic activity

• Enzyme Commision number: 5.6.2.1: DNA topoisomerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	H266 R364 V410 T411 W412 Y426 M428 K439 K443 R532 D533 K587 K650 N722 F723	H65 R163 V208 T209 W210 Y224 M226 K237 K241 R330 D331 K385 K438 N505 F506
BS02	dna	A	E356 F361 R364 H367 K374 K425 R488 A489 G490 N491 R532 N574 Q578 T585 A586 K587 R708 N745 K746 T747	E155 F160 R163 H166 K173 K223 R286 A287 G288 N289 R330 N372 Q376 T383 A384 K385 R496 N528 K529 T530

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003917 DNA topoisomerase type I (single strand cut, ATP-independent) activity

Biological Process

• GO:0006265 DNA topological change

Cellular Component

• GO:0005694 chromosome

External links

• PDB: RCSB:1r49, PDBe:1r49, PDBj:1r49
• PDBsum: 1r49
• PubMed: 14594810
• UniProt: P11387|TOP1_HUMAN DNA topoisomerase 1 (Gene Name=TOP1)