Structure of PDB 1qps Chain A

Receptor sequence
>1qpsA (length=261) Species: 562 (Escherichia coli) [Search protein sequence]
SQGVIGIFGDYAKAHDLAVGEVSKLVKKALSNEYPQLSFRYRDSIKKTEI
NEALKKIDPDLGGTLFVSNSSIKPDGGIVEVKDDYGEWRVVLVAEAKHQG
KDIINIRNGLLVGKRGDQDLMAAGNAIERSHKNISEIANFMLSESHFPYV
LFLEGSNFLTENISITRPDGRVVNLEYNSGILNRLDRLTAANYGMPINSN
LCINKFVNHKDKSIMLQAASIYTQGDGREWDSKIMFEIMFDISTTSLRVL
GRDLFEQLTSK
3D structure
PDB1qps The Integration of Recognition and Cleavage: X-Ray Structures of Pre- Transition State and Post-Reactive DNA-Eco RI Endonuclease Complexes
ChainA
Resolution2.5 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A V83 N85 S86 S87 I88 K89 N149 V67 N69 S70 S71 I72 K73 N133
BS02 dna A K113 Q115 K117 G129 K130 M137 A138 A139 G140 A142 R145 K97 Q99 K101 G113 K114 M121 A122 A123 G124 A126 R129
BS03 MN A D91 E111 A112 D75 E95 A96
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:1qps, PDBe:1qps, PDBj:1qps
PDBsum1qps
PubMed
UniProtP00642|T2E1_ECOLX Type II restriction enzyme EcoRI (Gene Name=ecoRIR)

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