Structure of PDB 1q1a Chain A

Receptor sequence

>1q1aA (length=285) Species: 4932 (Saccharomyces cerevisiae)

TASTEMSVRKIAAHMKSNPNAKVIFMVGAGISTSCGIPDFRSPGTGLYHN
LARLKLPYPEAVFDVDFFQSDPLPFYTLAKELYPGNFRPSKFHYLLKLFQ
DKDVLKRVYTQNIDTLERQAGVKDDLIIEAHGSFAHCHCIGCGKVYPPQV
FKSKLAEHPIKDFVKCDVCGELVKPAIVFFGEDLPDSFSETWLNDSEWLR
EKITTPQQPLVIVVGTSLAVYPFASLPEEIPRKVKRVLCNLETVGDFKAN
KRPTDLIVHQYSDEFAEQLVEELGWQEDFEKILTA

3D structure

• PDB: 1q1a Structure of the yeast Hst2 protein deacetylase in ternary complex with 2'-O-Acetyl ADP ribose and histone peptide.
• Chain: A
• Resolution: 1.5 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): P42 D43 F44 R45 N116 D118 H135
• Catalytic site (residue number reindexed from 1): P38 D39 F40 R41 N112 D114 H131
• Enzyme Commision number: 2.3.1.286: protein acetyllysine N-acetyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	A	E64 F67 H135 V182 F184 G185 E186 D187 L226 A227 V228 Y229 P230 S233 E237	E60 F63 H131 V178 F180 G181 E182 D183 L218 A219 V220 Y221 P222 S225 E229
BS02	ZN	A	C143 C146 C170 C173	C139 C142 C166 C169
BS03	OAD	A	G32 G34 T37 D43 F44 R45 Y52 Q115 H135 G223 T224 S225 N248 L249 Y269 S270	G28 G30 T33 D39 F40 R41 Y48 Q111 H131 G215 T216 S217 N240 L241 Y261 S262

Gene Ontology

Molecular Function

• GO:0017136 NAD-dependent histone deacetylase activity
• GO:0051287 NAD binding
• GO:0070403 NAD+ binding

External links

• PDB: RCSB:1q1a, PDBe:1q1a, PDBj:1q1a
• PDBsum: 1q1a
• PubMed: 14604530
• UniProt: P53686|HST2_YEAST NAD-dependent protein deacetylase HST2 (Gene Name=HST2)