Structure of PDB 1pwu Chain A

Receptor sequence
>1pwuA (length=747) Species: 1392 (Bacillus anthracis) [Search protein sequence]
KTQEEHLKEIMKHIVKIEVKGEEAVKKEAAEKLLEKVPSDVLEMYKAIGG
KIYIVDGDITKHISLEALSEDKKKIKDIYGKDALLHEHYVYAKEGYEPVL
VIQSSEDYVENTEKALNVYYEIGKILSRDILSKINQPYQKFLDVLNTIKN
ASDSDGQDLLFTNQLKEHPTDFSVEFLEQNSNEVQEVFAKAFAYYIEPQH
RDVLQLYAPEAFNYMDKFNEQEINLSLEELKDQRMLSRYEKWEKIKQHYQ
HWSDSLSEEGRGLLKKLQIPIEPKKDDIIHSLSQEEKELLKRIQIDSSDF
LSTEEKEFLKKLQIDIRDSLSEEEKELLNRIQVDSSNPLSEKEKEFLKKL
KLDIQPYDINQRLQDTGGLIDSPSINLDVRKQYKRDIQNIDALLHQSIGS
TLYNKIYLYENMNINNLTATLGADLVDSTDNTKINRGIFNEFKKNFKYSI
SSNYMIVDINERPALDNERLKWRIQLSPDTRAGYLENGKLILQRNIGLEI
KDVQIIKQSEKEYIRIDAKVVPKSKIDTKIQEAQLNINQEWNKALGLPKY
TKLITFNVHNRYASNIVESAYLILNEWKNNIQSDLIKKVTNYLVDGNGRF
VFTDITLPNIAEQYTHQDEIYEQVHSKGLYVPESRSILLHGPSKGVELRN
DSEGFIHCFGHAVDDYAGYLLDKNQSDLVTNSKKFIDIFKEEGSNLTSYG
RTNEAEFFAEAFRLMHSTDHAERLKVQKNAPKTFQFINDQIKFIINS
3D structure
PDB1pwu The structural basis for substrate and inhibitor selectivity of the anthrax lethal factor.
ChainA
Resolution2.7 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) H686 C687 H690 Y728 E735
Catalytic site (residue number reindexed from 1) H657 C658 H661 Y699 E706
Enzyme Commision number 3.4.24.83: anthrax lethal factor endopeptidase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ZN A H686 H690 E735 H657 H661 E706
BS02 GM6 A D328 H654 S655 K656 G657 G674 V675 L677 H686 H690 Y728 D299 H625 S626 K627 G628 G645 V646 L648 H657 H661 Y699 PDBbind-CN: -logKd/Ki=5.68,Ki=2.1uM
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0004222 metalloendopeptidase activity
GO:0005515 protein binding
GO:0008237 metallopeptidase activity
GO:0008270 zinc ion binding
GO:0046872 metal ion binding
GO:0090729 toxin activity
Biological Process
GO:0006508 proteolysis
GO:0035821 modulation of process of another organism
Cellular Component
GO:0005576 extracellular region
GO:0030430 host cell cytoplasm
GO:0044164 host cell cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1pwu, PDBe:1pwu, PDBj:1pwu
PDBsum1pwu
PubMed14718924
UniProtP15917|LEF_BACAN Lethal factor (Gene Name=lef)

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