Structure of PDB 1pjs Chain A

Receptor sequence
>1pjsA (length=443) [Search protein sequence]
MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLEAGARLTVNALTFIPQFT
VWANEGMLTLVEGPFDETLLDSCWLAIAATDDDTVNQRVSDAAESRRIFC
NVVDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPQH
LGQVARYAGQLRARVKKQFATMGERRRFWEKFFVNDRLAQSLANADEKAV
NATTERLFSEPLDHRGEVVLVGAGPGDAGLLTLKGLQQIQQADIVVYDRL
VSDDIMNLVRRDADRVFVVPQEEINQILLREAQKGKRVVRLKGGDPFIFG
RGGEELETLCHAGIPFSVVPGITAASGCSAYSGIPLTHRDYAQSVRLVTG
ELDWENLAAEKQTLVFYMGLNQAATIQEKLIAFGMQADMPVALVENGTSV
KQRVVHGVLTQLGELAQQVESPALIIVGRVVALRDKLNWFSNH
3D structure
PDB1pjs CysG structure reveals tetrapyrrole-binding features and novel regulation of siroheme biosynthesis.
ChainA
Resolution2.4 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D248 M382
Catalytic site (residue number reindexed from 1) D248 M368
Enzyme Commision number 1.3.1.76: precorrin-2 dehydrogenase.
2.1.1.107: uroporphyrinogen-III C-methyltransferase.
4.99.1.4: sirohydrochlorin ferrochelatase.
Interaction with ligand
Site
#
Ligand Ligand
chain
Binding residues on receptor
(original residue number in PDB)
Binding residues on receptor
(residue number reindexed from 1)
Binding affinity
BS01 SAH A G301 G302 D303 I306 T331 A332 C336 Y381 M382 N410 G411 A437 G293 G294 D295 I298 T323 A324 C328 Y367 M368 N396 G397 A423
BS02 NAD A G20 G21 D22 V23 A42 L43 T44 T80 D81 V85 G20 G21 D22 V23 A42 L43 T44 T80 D81 V85
Gene Ontology
Molecular Function
GO:0004325 ferrochelatase activity
GO:0004851 uroporphyrin-III C-methyltransferase activity
GO:0008168 methyltransferase activity
GO:0016491 oxidoreductase activity
GO:0016829 lyase activity
GO:0043115 precorrin-2 dehydrogenase activity
GO:0051266 sirohydrochlorin ferrochelatase activity
GO:0051287 NAD binding
Biological Process
GO:0006779 porphyrin-containing compound biosynthetic process
GO:0009236 cobalamin biosynthetic process
GO:0019354 siroheme biosynthetic process
GO:0032259 methylation

View graph for
Molecular Function

View graph for
Biological Process
External links
PDB RCSB:1pjs, PDBe:1pjs, PDBj:1pjs
PDBsum1pjs
PubMed14595395
UniProtP25924|CYSG_SALTY Siroheme synthase (Gene Name=cysG)

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