Structure of PDB 1p9p Chain A

Receptor sequence
>1p9pA (length=235) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHDRHRTVD
DRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVS
ELATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLID
SVSRFIPGVLGEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQ
SLGRTWLRRPELLENLALTEEQARLLAEFKTEHAQ
3D structure
PDB1p9p Insights into Catalysis by a Knotted TrmD tRNA Methyltransferase.
ChainA
Resolution2.5 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) P89 E116 R154
Catalytic site (residue number reindexed from 1) P89 E116 R154
Enzyme Commision number 2.1.1.228: tRNA (guanine(37)-N(1))-methyltransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 SAH A Y86 L87 S88 P89 Q90 G113 Y115 I133 L138 G140 G141 P144 Y86 L87 S88 P89 Q90 G113 Y115 I133 L138 G140 G141 P144 MOAD: Kd=68nM
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0008168 methyltransferase activity
GO:0042802 identical protein binding
GO:0042803 protein homodimerization activity
GO:0052906 tRNA (guanine(37)-N1)-methyltransferase activity
Biological Process
GO:0002939 tRNA N1-guanine methylation
GO:0006400 tRNA modification
GO:0008033 tRNA processing
GO:0030488 tRNA methylation
GO:0032259 methylation
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1p9p, PDBe:1p9p, PDBj:1p9p
PDBsum1p9p
PubMed14583191
UniProtP0A873|TRMD_ECOLI tRNA (guanine-N(1)-)-methyltransferase (Gene Name=trmD)

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