Structure of PDB 1p6h Chain A

Receptor sequence

>1p6hA (length=407) Species: 10116 (Rattus norvegicus)

RFLKVKNWETDVVLTDTLHLKSTLETGCTEHICMGSIMLPTKDQLFPLAK
EFLDQYYSSIKRFGSKAHMDRLEEVNKEIESTSTYQLKDTELIYGAKHAW
RNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAI
TIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVQFTEICIQQG
WKAPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFDWFKDLGL
KWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEE
VAKKMDLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFI
KHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPD
PWNTHVW

3D structure

• PDB: 1p6h Structural basis for dipeptide amide isoform-selective inhibition of neuronal nitric oxide synthase.
• Chain: A
• Resolution: 1.98 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C415 R418 W587 E592
• Catalytic site (residue number reindexed from 1): C106 R109 W278 E283
• Enzyme Commision number: 1.14.13.39: nitric-oxide synthase (NADPH).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	A	C326 C331	C28 C33
BS02	HEM	A	W409 C415 F584 S585 W587 E592 W678 F704 Y706	W100 C106 F275 S276 W278 E283 W369 F395 Y397
BS03	H4B	A	S334 R596 V677 W678	S36 R287 V368 W369
BS04	DP1	A	S477 Q478 R481 P565 V567 S585 G586 W587 E592	S168 Q169 R172 P256 V258 S276 G277 W278 E283	MOAD: Ki=0.3uM BindingDB: Ki=130nM
BS05	H4B	A	F691 H692 E694	F382 H383 E385

Gene Ontology

Molecular Function

• GO:0004517 nitric-oxide synthase activity

Biological Process

• GO:0006809 nitric oxide biosynthetic process

External links

• PDB: RCSB:1p6h, PDBe:1p6h, PDBj:1p6h
• PDBsum: 1p6h
• PubMed: 14718923
• UniProt: P29476|NOS1_RAT Nitric oxide synthase 1 (Gene Name=Nos1)