Structure of PDB 1p4e Chain A

Receptor sequence

>1p4eA (length=400) Species: 4932 (Saccharomyces cerevisiae)

SQFDILCKTPPKVLVRQFVERFERPSGEKIASCAAELTYLCWMITHNGTA
IKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWE
FTIIPYNGQSDITDIVSSLQLQFESGNSHSKKMLKALLSEGESIWEITEK
ILNSFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKY
LGVIIQCLVTETKTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVN
RTGNSSSNKQEYQLLKDNLVRSYNKALKKNAPYPIFAIKNGPKSHIGRHL
MTSFLSMKGLTELTNVVGNFSDKQITAIPDHYFALVSRYYAYDPISKEMI
ALKDETNPIEEWQHIEQLKGSAEGSIRYPAWNGIISQEVLDYLSSYINRR

3D structure

• PDB: 1p4e The role of the conserved Trp330 in Flp-mediated recombination. Functional and structural analysis
• Chain: A
• Resolution: 2.7 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	S2 Q3 M58 T62 S169 R170 T174 Y178 K223 N278 R281 K285 K289 N300	S1 Q2 M57 T61 S159 R160 T164 Y168 K213 N268 R271 K275 K279 N290
BS02	dna	A	R191 T224 F330 S331	R181 T214 F320 S321
BS03	dna	A	H47 K53 A55 T56 Y60 K82 N108 F192 S193 K223 R281 N284 K299 N300 G301 K303 S304 H305	H46 K52 A54 T55 Y59 K81 N107 F182 S183 K213 R271 N274 K289 N290 G291 K293 S294 H295

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003690 double-stranded DNA binding
• GO:0003697 single-stranded DNA binding
• GO:0008301 DNA binding, bending
• GO:0009009 site-specific recombinase activity

Biological Process

• GO:0006310 DNA recombination
• GO:0015074 DNA integration
• GO:0042150 plasmid recombination

Cellular Component

• GO:0005575 cellular_component

External links

• PDB: RCSB:1p4e, PDBe:1p4e, PDBj:1p4e
• PDBsum: 1p4e
• PubMed: 12716882
• UniProt: P03870|FLP_YEAST Site-specific recombinase Flp (Gene Name=FLP1)