Structure of PDB 1p33 Chain A

Receptor sequence

>1p33A (length=267) Species: 5689 (Leishmania tarentolae)

TAPVALVTGAAKRLGSSIAEALHAEGYTVCLHYHRSAADASTLAATLNAR
RPNSAITVQADLSNVATASSVPVTLFSRCSALVDACYMHWGRCDVLVNNA
SSFYPTPLLRDKESLEVAAADLFGSNAIAPYFLIKAFAQRVADTRAEQRG
TSYSIVNMVDAMTSQPLLGYTMYTMAKEALEGLTRSAALELASLQIRVNG
VSPGLSVLPDDMPFSVQEDYRRKVPLYQRNSSAEEVSDVVIFLCSPKAKY
ITGTCIKVDGGYSLTRA

3D structure

• PDB: 1p33 Structure of pteridine reductase (PTR1) from Leishmania tarentolae.
• Chain: A
• Resolution: 2.86 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): R17 D181 Y194
• Catalytic site (residue number reindexed from 1): R13 D160 Y173
• Enzyme Commision number: 1.5.1.33: pteridine reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NDP	A	R17 L18 H38 R39 S40 D65 L66 N109 A110 S111 S112 D142 S146 M179 V180 D181 K198 P224 G225 S227	R13 L14 H34 R35 S36 D61 L62 N99 A100 S101 S102 D121 S125 M158 V159 D160 K177 P203 G204 S206
BS02	MTX	A	R17 S111 F113 L188 Y191 Y194 P230 Y241	R13 S101 F103 L167 Y170 Y173 P209 Y220

Gene Ontology

Molecular Function

• GO:0016491 oxidoreductase activity
• GO:0047040 pteridine reductase activity

Biological Process

• GO:0006729 tetrahydrobiopterin biosynthetic process
• GO:0031427 response to methotrexate

External links

• PDB: RCSB:1p33, PDBe:1p33, PDBj:1p33
• PDBsum: 1p33
• PubMed: 12925782
• UniProt: P42556|PTR1_LEITA Pteridine reductase 1 (Gene Name=PTR1)