Structure of PDB 1p1j Chain A

Receptor sequence

>1p1jA (length=525) Species: 4932 (Saccharomyces cerevisiae)

ITSVKVVTDKCTYKDNELLTKYSYENAVVTKTASGRFDVTPTVQDYVFKL
DLKKPEKLGIMLIGLGGNNGSTLVASVLANKHNVEFQTKEGVKQPNYFGS
MTQCSTLKLGIDAEGNDVYAPFNSLLPMVSPNDFVVSGWDINNADLYEAM
QRSQVLEYDLQQRLKAKMSLVKPLPSIYYPDFIAANQDERANNCINLDEK
GNVTTRGKWTHLQRIRRDIQNFKEENALDKVIVLWTANTERYVEVSPGVN
DTMENLLQSIKNDHEEIAPSTIFAAASILEGVPYINGSPQNTFVPGLVQL
AEHEGTFIAGDDLKSGQTKLKSVLAQFLVDAGIKPVSIASYNHLGNNDGY
NLSAPKQFRSKEISKSSVIDDIIASNDILYNDKLGKKVDHCIVIKYMKPV
GDSKVAMDEYYSELMLGGHNRISIHNVCEDSLLATPLIIDLLVMTEFCTR
VSYKKVDPVKEDAGKFENFYPVLTFLSYWLKAPLTRPGFHPVNGLNKQRT
ALENFLRLLIGLPSQNELRFEERLL

3D structure

• PDB: 1p1j Structures of NAD(+)- and NADH-bound 1-l-myo-inositol 1-phosphate synthase.
• Chain: A
• Resolution: 1.7 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D320 K369 K412 K489
• Catalytic site (residue number reindexed from 1): D312 K361 K404 K481
• Enzyme Commision number: 5.5.1.4: inositol-3-phosphate synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	PO4	A	G324 Q325 T326 K373 K412 K489	G316 Q317 T318 K365 K404 K481
BS02	NAI	A	I71 G72 G75 N76 N77 D148 I149 S184 I185 R198 T244 A245 N246 T247 D320 L321 S323 N355 D356 K369 D438 A442	I63 G64 G67 N68 N69 D140 I141 S176 I177 R190 T236 A237 N238 T239 D312 L313 S315 N347 D348 K361 D430 A434
BS03	GOL	A	N350 D356 K369 K373 I402 K412 D438	N342 D348 K361 K365 I394 K404 D430

Gene Ontology

Molecular Function

• GO:0004512 inositol-3-phosphate synthase activity
• GO:0016853 isomerase activity

Biological Process

• GO:0006021 inositol biosynthetic process
• GO:0008654 phospholipid biosynthetic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:1p1j, PDBe:1p1j, PDBj:1p1j
• PDBsum: 1p1j
• PubMed: 12832758
• UniProt: P11986|INO1_YEAST Inositol-3-phosphate synthase (Gene Name=INO1)