Structure of PDB 1p0f Chain A

Receptor sequence

>1p0fA (length=372) Species: 8403 (Pelophylax perezi)

CTAGKDITCKAAVAWEPHKPLSLETITVAPPKAHEVRIKILASGICGSDS
SVLKEIIPSKFPVILGHEAVGVVESIGAGVTCVKPGDKVIPLFVPQCGSC
RACKSSNSNFCEKNDMGAKTGLMADMTSRFTCRGKPIYNLMGTSTFTEYT
VVADIAVAKIDPKAPLESCLIGCGFATGYGAAVNTAKVTPGSTCAVFGLG
GVGFSAIVGCKAAGASRIIGVGTHKDKFPKAIELGATECLNPKDYDKPIY
EVICEKTNGGVDYAVECAGRIETMMNALQSTYCGSGVTVVLGLASPNERL
PLDPLLLLTGRSLKGSVFGGFKGEEVSRLVDDYMKKKINVNFLVSTKLTL
DQINKAFELLSSGQGVRSIMIY

3D structure

• PDB: 1p0f Crystal structure of the vertebrate NADP(H)-dependent alcohol dehydrogenase (ADH8)
• Chain: A
• Resolution: 1.8 Å

Enzymatic activity

• Enzyme Commision number: 1.1.1.2: alcohol dehydrogenase (NADP(+)).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	A	C1046 H1067 C1173	C46 H67 C173
BS02	ZN	A	C1097 C1100 C1103 C1111	C97 C100 C103 C111
BS03	NAP	A	C1046 G1047 S1048 C1173 T1177 G1198 L1199 G1200 G1201 V1202 G1222 T1223 H1224 K1227 C1267 A1268 R1270 L1291 G1292 L1293 V1317 R1367	C46 G47 S48 C173 T177 G198 L199 G200 G201 V202 G222 T223 H224 K227 C267 A268 R270 L291 G292 L293 V317 R367

Gene Ontology

Molecular Function

• GO:0004745 all-trans-retinol dehydrogenase (NAD+) activity
• GO:0008106 alcohol dehydrogenase (NADP+) activity
• GO:0008270 zinc ion binding
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0042572 retinol metabolic process
• GO:0042573 retinoic acid metabolic process

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:1p0f, PDBe:1p0f, PDBj:1p0f
• PDBsum: 1p0f
• PubMed: 12818203
• UniProt: O57380|ADH8_PELPE NADP-dependent alcohol dehydrogenase (Gene Name=ADH8)