Structure of PDB 1os1 Chain A

Receptor sequence
>1os1A (length=537) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
NNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGYERGVLTNLG
AVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETWQ
HLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFI
RPSDEELAGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIG
GTWYGGEMKKGMFSMMNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTG
KTTLSTDPKRRLIGDDEHGWDDDGVFNFEGGCYAKTIKLSKEAEPEIYNA
IRRDALLENVTVREDGTIDFDDGSKTENTRVSYPIYHIDNIVKPVSKAGH
ATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLAGTERGITEPTP
TFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRISIK
DTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASP
EQWQEKAETLAKLFIDNFDKYTDTPAGAALVAAGPKL
3D structure
PDB1os1 Mechanisms of activation of phosphoenolpyruvate carboxykinase from Escherichia coli by Ca2+ and of desensitization by trypsin.
ChainA
Resolution1.8 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R65 K213 H232 S250 K254 T255 D269 R333
Catalytic site (residue number reindexed from 1) R62 K210 H229 S247 K251 T252 D266 R330
Enzyme Commision number 4.1.1.49: phosphoenolpyruvate carboxykinase (ATP).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 CA A K213 H232 D269 K210 H229 D266
BS02 ATP A L249 S250 G251 T252 G253 K254 T255 T256 R333 R449 S451 I452 T455 L246 S247 G248 T249 G250 K251 T252 T253 R330 R446 S448 I449 T452
BS03 PYR A R65 Y207 K213 R62 Y204 K210
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0004611 phosphoenolpyruvate carboxykinase activity
GO:0004612 phosphoenolpyruvate carboxykinase (ATP) activity
GO:0005509 calcium ion binding
GO:0005524 ATP binding
GO:0016831 carboxy-lyase activity
GO:0017076 purine nucleotide binding
GO:0046872 metal ion binding
Biological Process
GO:0006094 gluconeogenesis
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Molecular Function
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Cellular Component
External links
PDB RCSB:1os1, PDBe:1os1, PDBj:1os1
PDBsum1os1
PubMed12837799
UniProtP22259|PCKA_ECOLI Phosphoenolpyruvate carboxykinase (ATP) (Gene Name=pckA)

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