Structure of PDB 1olu Chain A

Receptor sequence
>1oluA (length=366) Species: 9606 (Homo sapiens) [Search protein sequence]
PQFPGASAEFIDKLEFIQPNGIPIYRVMDRQGQIINPSEDPHLPKEKVLK
LYKSMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALDNTDLV
FGQYREAGVLMYRDYPLELFMAQCYGNISDLGKGRQMPVHYGCKERHFVT
ISSPLATQIPQAVGAAYAAKRANANRVVICYFGEGAASEGDAHAGFNFAA
TLECPIIFFCRNNGYAISTPTSEQYRGDGIAARGPGYGIMSIRVDGNDVF
AVYNATKEARRRAVAENQPFLIEAMTYRDHPISRLRHYLLSQGWWDEEQE
KAWRKQSRRKVMEAFEQAERKPKPNPNLLFSDVYQEMPAQLRKQQESLAR
HLQTYGEHYPLDHFDK
3D structure
PDB1olu Roles of His291-Alpha and His146-Beta' in the Reductive Acylation Reaction Catalyzed by Human Branched-Chain Alpha-Ketoacid Dehydrogenase: Refined Phosphorylation Loop Structure in the Active Site.
ChainA
Resolution1.9 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) E76 S162 R287
Catalytic site (residue number reindexed from 1) E67 S153 R278
Enzyme Commision number 1.2.4.4: 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG A E193 N222 Y224 E184 N213 Y215
BS02 TPP A R114 L164 G192 E193 G194 A195 N222 A225 I226 R105 L155 G183 E184 G185 A186 N213 A216 I217
Gene Ontology
Molecular Function
GO:0003863 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity
GO:0005515 protein binding
GO:0016491 oxidoreductase activity
GO:0016624 oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor
GO:0016831 carboxy-lyase activity
GO:0046872 metal ion binding
GO:0047101 branched-chain alpha-keto acid dehydrogenase activity
Biological Process
GO:0009083 branched-chain amino acid catabolic process
Cellular Component
GO:0005739 mitochondrion
GO:0005759 mitochondrial matrix
GO:0045252 oxoglutarate dehydrogenase complex
GO:0160157 branched-chain alpha-ketoacid dehydrogenase complex

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Molecular Function
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Cellular Component
External links
PDB RCSB:1olu, PDBe:1olu, PDBj:1olu
PDBsum1olu
PubMed12902323
UniProtP12694|ODBA_HUMAN 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial (Gene Name=BCKDHA)

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