Structure of PDB 1of0 Chain A

Receptor sequence

>1of0A (length=502) Species: 1423 (Bacillus subtilis)

TLEKFVDALPIPDTLKPVQQSKEKTYYEVTMEECTHQLHRDLPPTRLWGY
NGLFPGPTIEVKRNENVYVKWMNNLPSTHFLPIDHTIHEPEVKTVVHLHG
GVTPDDSDGYPEAWFSKDFEQTGPYFKREVYHYPNQQRGAILWYHDHAMA
LTRLNVYAGLVGAYIIHDPKEKRLKLPSDEYDVPLLITDRTINEDGSLFY
PSAPENPSPSLPNPSIVPAFCGETILVNGKVWPYLEVEPRKYRFRVINAS
NTRTYNLSLDNGGDFIQIGSDGGLLPRSVKLNSFSLAPAERYDIIIDFTA
YEGESIILANSAGCGGDVNPETDANIMQFRVTKPLAQKDESRKPKYLASY
PSVQHERIQNIRTLKLAGTQDEYGRPVLLLNNKRWHDPVTETPKVGTTEI
WSIINPTRGTHPIHLHLVSFRVLDRRPFDIARYQESGELSYTGPAVPPPP
SEKGWKDTIQAHAGEVLRIAATFGPYSGRYVWHCHILEHEDYDMMRPMDI
TD

3D structure

• PDB: 1of0 Structural Characterization of a Bacterial Laccase Reaction Cycle
• Chain: A
• Resolution: 2.45 Å

Enzymatic activity

• Enzyme Commision number: 1.10.3.2: laccase.
  1.3.3.5: bilirubin oxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CU1	A	H419 C492 H497 D510	H411 C484 H489 D502
BS02	CU1	A	H107 H153 H493	H99 H145 H485
BS03	CU1	A	H155 H424 H491	H147 H416 H483
BS04	OXY	A	H153 H155 H491 H493 E498	H145 H147 H483 H485 E490
BS05	EBS	A	P226 A227 C322 G323 P384 L386 T415 G417 H419 H497	P218 A219 C314 G315 P376 L378 T407 G409 H411 H489

Gene Ontology

Molecular Function

• GO:0005507 copper ion binding
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0030435 sporulation resulting in formation of a cellular spore

Cellular Component

• GO:0030288 outer membrane-bounded periplasmic space

External links

• PDB: RCSB:1of0, PDBe:1of0, PDBj:1of0
• PDBsum: 1of0
• UniProt: P07788|COTA_BACSU Laccase (Gene Name=cotA)