Structure of PDB 1o95 Chain A

Receptor sequence

>1o95A (length=729) Species: 17 (Methylophilus methylotrophus)

ARDPKHDILFEPIQIGPKTLRNRFYQVPHCIGAGSDKPGFQSAHRSVKAE
GGWAALNTEYCSINPESDDTHRLSARIWDEGDVRNLKAMTDEVHKYGALA
GVELWYGGAHAPNMESRATPRGPSQYASEFETLSYCKEMDLSDIAQVQQF
YVDAAKRSRDAGFDIVYVYGAHSYLPLQFLNPYYNKRTDKYGGSLENRAR
FWLETLEKVKHAVGSDCAIATRFGVDTVYGPGQIEAEVDGQKFVEMADSL
VDMWDITIGDIAEWGEDAGPSRFYQQGHTIPWVKLVKQVSKKPVLGVGRY
TDPEKMIEIVTKGYADIIGCARPSIADPFLPQKVEQGRYDDIRVCIGCNV
CISRWEIGGPPMICTQNATAGEEYRRGWHPEKFRQTKNKDSVLIVGAGPS
GSEAARVLMESGYTVHLTDTAEKIGGHLNQVAALPGLGEWSYHRDYRETQ
ITKLLKKNKESQLALGQKPMTADDVLQYGADKVIIATGARWNTDGTNCLT
HDPIPGADASLPDQLTPEQVMDGKKKIGKRVVILNADTYFMAPSLAEKLA
TAGHEVTIVSGVHLANYMHFTLEYPNMMRRLHELHVEELGDHFCSRIEPG
RMEIYNIWGDGSKRTYRGPGVSPRDANTSHRWIEFDSLVLVTGRHSECTL
WNELKARESEWAENDIKGIYLIGDAEAPRLIADATFTGHRVAREIEEANP
QIAIPYKRETIAWGTPHMPGGNFKIEYKV

3D structure

• PDB: 1o95 Extensive Conformational Sampling in a Ternary Electron Transfer Complex.
• Chain: A
• Resolution: 3.7 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C30 Y169 H172 Y174 R222 Y229 D267 V350
• Catalytic site (residue number reindexed from 1): C30 Y169 H172 Y174 R222 Y229 D267 V350
• Enzyme Commision number: 1.5.8.2: trimethylamine dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FMN	A	P28 H29 C30 Y60 E103 Y169 H172 R222 W264 D267 A268 V297 R299 C320 A321 R322 I352	P28 H29 C30 Y60 E103 Y169 H172 R222 W264 D267 A268 V297 R299 C320 A321 R322 I352
BS02	ADP	A	V395 G396 G398 P399 S400 D419 G426 H427 P469 M470 A486 T487 G488 G673 D674	V395 G396 G398 P399 S400 D419 G426 H427 P469 M470 A486 T487 G488 G673 D674
BS03	SF4	A	R322 I325 C345 I346 G347 C348 N349 V350 C351 C364 T365	R322 I325 C345 I346 G347 C348 N349 V350 C351 C364 T365

Gene Ontology

Molecular Function

• GO:0010181 FMN binding
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0050470 trimethylamine dehydrogenase activity
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0009056 catabolic process

External links

• PDB: RCSB:1o95, PDBe:1o95, PDBj:1o95
• PDBsum: 1o95
• PubMed: 12567183
• UniProt: P16099|DHTM_METME Trimethylamine dehydrogenase (Gene Name=tmd)