Structure of PDB 1o0t Chain A

Receptor sequence

>1o0tA (length=455) Species: 76981 (Pseudomonas sp. 'TAC II 18')

GTSSAFTQIDNFSHFYDRGDHLVNGKPSFTVDQVADQLTRSGASWHDLNN
DGVINLTYTFLTAPPVGYASRGLGTFSQFSALQKEQAKLSLESWADVAKV
TFTEGPAARGDDGHMTFANFSASNGGAAFAYLPNSSRKGESWYLINKDYQ
VNKTPGEGNYGRQTLTHEIGHTLGLSHPGDNPTYRDAVYAEDTRAYSVMS
YWSEKNTGQVFTKTGEGAYASAPLLDDIAAVQKLYGANLETRADDTVYGF
NSTADRDFYSATSSTDKLIFSVWDGGGNDTLDFSGFSQNQKINLTAGSFS
DVGGMTGNVSIAQGVTIENAIGGSGNDLLIGNDAANVLKGGAGNDIIYGG
GGADVLWGGTGSDTFVFGAVSDSTPKAADIIKDFQSGFDKIDLTAITKLG
GLNFVDAFTGHAGDAIVSYHQASNAGSLQVDFSGQGVADFLVTTVGQVAT
YDIVA

3D structure

• PDB: 1o0t Probing the role of divalent metal ions in a bacterial psychrophilic metalloprotease: binding studies of an enzyme in the crystalline state by x-ray crystallography.
• Chain: A
• Resolution: 2.5 Å

Enzymatic activity

• Enzyme Commision number: 3.4.24.40: serralysin.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	A	D49 N51 D53 V55 N57 D114	D47 N49 D51 V53 N55 D112
BS02	CA	A	R250 D252 T254 D282 G284 D287	R242 D244 T246 D274 G276 D279
BS03	CA	A	G284 G285 D287 T324 E326	G276 G277 D279 T316 E318
BS04	CA	A	G331 G333 D335 G348 G349 A350 D353	G323 G325 D327 G340 G341 A342 D345
BS05	CA	A	G349 A350 G351 D353 G366 T368 D371	G341 A342 G343 D345 G358 T360 D363
BS06	CA	A	N340 A342 N344 G357 G358 G359 D362	N332 A334 N336 G349 G350 G351 D354
BS07	CA	A	G358 G360 D362 D380 D387	G350 G352 D354 D372 D379

Gene Ontology

Molecular Function

• GO:0004222 metalloendopeptidase activity
• GO:0005509 calcium ion binding
• GO:0008237 metallopeptidase activity
• GO:0008270 zinc ion binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006508 proteolysis

Cellular Component

• GO:0005615 extracellular space
• GO:0031012 extracellular matrix

External links

• PDB: RCSB:1o0t, PDBe:1o0t, PDBj:1o0t
• PDBsum: 1o0t
• PubMed: 12837794
• UniProt: O69771