Structure of PDB 1o0s Chain A

Receptor sequence
>1o0sA (length=602) Species: 6253 (Ascaris suum) [Search protein sequence]
SVAHHEDVYSHNLPPMDEKEMALYKLYRPERVTPKKRSAELLKEPRLNKG
MGFSLYERQYLGLHGLLPPAFMTQEQQAYRVITKLREQPNDLARYIQLDG
LQDRNEKLFYRVVCDHVKELMPIVYTPTVGLACQNFGYIYRKPKGLYITI
NDNSVSKIYQILSNWHEEDVRAIVVTDGERILGLGDLGAYGIGIPVGKLA
LYVALGGVQPKWCLPVLLDVGTNNMDLLNDPFYIGLRHKRVRGKDYDTLL
DNFMKACTKKYGQKTLIQFEDFANPNAFRLLDKYQDKYTMFNDDIQGTAS
VIVAGLLTCTRVTKKLVSQEKYLFFGAGAASTGIAEMIVHQMQNEGISKE
EACNRIYLMDIDGLVTKNRKEMNPRHVQFAKDMPETTSILEVIRAARPGA
LIGASTVRGAFNEEVIRAMAEINERPIIFALSNPTSKAECTAEEAYTFTN
GAALYASGSPFPNFELNGHTYKPGQGNNAYIFPGVALGTILFQIRHVDND
LFLLAAKKVASCVTEDSLKVGRVYPQLKEIREISIQIAVEMAKYCYKNGT
ANLYPQPEDLEKYVRAQVYNTEYEELINATYDWPEQDMRHGFPVPVVRHD
SM
3D structure
PDB1o0s Crystallographic studies on Ascaris suum NAD-malic enzyme bound to reduced cofactor and identification of an effector site.
ChainA
Resolution2.0 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) Y126 R181 K199 E271 D272 D294 D295 N434
Catalytic site (residue number reindexed from 1) Y125 R180 K198 E270 D271 D293 D294 N433
Enzyme Commision number 1.1.1.38: malate dehydrogenase (oxaloacetate-decarboxylating).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 TTN A Q78 R81 R105 L109 Q77 R80 R104 L108
BS02 NAI A R181 N275 D295 T299 F326 G327 A328 G329 A330 D361 I362 V408 L432 S433 N434 R180 N274 D294 T298 F325 G326 A327 G328 A329 D360 I361 V407 L431 S432 N433
Gene Ontology
Molecular Function
GO:0004470 malic enzyme activity
GO:0004471 malate dehydrogenase (decarboxylating) (NAD+) activity
GO:0004473 malate dehydrogenase (decarboxylating) (NADP+) activity
GO:0008948 oxaloacetate decarboxylase activity
GO:0016491 oxidoreductase activity
GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0046872 metal ion binding
GO:0051287 NAD binding
Biological Process
GO:0006090 pyruvate metabolic process
GO:0006108 malate metabolic process
Cellular Component
GO:0005739 mitochondrion
GO:0005759 mitochondrial matrix

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1o0s, PDBe:1o0s, PDBj:1o0s
PDBsum1o0s
PubMed12853453
UniProtP27443|MAOM_ASCSU NAD-dependent malic enzyme, mitochondrial (Fragment)

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