Structure of PDB 1o0q Chain A

Receptor sequence

>1o0qA (length=453) Species: 76981 (Pseudomonas sp. 'TAC II 18')

GTSSAFTQIDNFSHFYDRGDHLVNGKPSFTVDQVADQLTRSGASWHDNDG
VINLTYTFLTAPPVGYASRGLGTFSQFSALQKEQAKLSLESWADVAKVTF
TEGPAARGDDGHMTFANFSASNGGAAFAYLPNSSRKGESWYLINKDYQVN
KTPGEGNYGRQTLTHEIGHTLGLSHPGDNPTYRDAVYAEDTRAYSVMSYW
SEKNTGQVFTKTGEGAYASAPLLDDIAAVQKLYGANLETRADDTVYGFNS
TADRDFYSATSSTDKLIFSVWDGGGNDTLDFSGFSQNQKINLTAGSFSDV
GGMTGNVSIAQGVTIENAIGGSGNDLLIGNDAANVLKGGAGNDIIYGGGG
ADVLWGGTGSDTFVFGAVSDSTPKAADIIKDFQSGFDKIDLTAITKLGGL
NFVDAFTGHAGDAIVSYHQASNAGSLQVDFSGQGVADFLVTTVGQVATYD
IVA

3D structure

• PDB: 1o0q Probing the role of divalent metal ions in a bacterial psychrophilic metalloprotease: binding studies of an enzyme in the crystalline state by x-ray crystallography.
• Chain: A
• Resolution: 2.2 Å

Enzymatic activity

• Enzyme Commision number: 3.4.24.40: serralysin.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	A	R250 D252 T254 D282 G284 D287	R240 D242 T244 D272 G274 D277
BS02	CA	A	G285 D287 T324 E326	G275 D277 T314 E316
BS03	CA	A	G331 G333 D335 G348 A350 D353	G321 G323 D325 G338 A340 D343
BS04	CA	A	G349 A350 G351 D353 G366 T368 D371	G339 A340 G341 D343 G356 T358 D361
BS05	CA	A	N340 A342 N344 G357 G358 G359 D362	N330 A332 N334 G347 G348 G349 D352
BS06	CA	A	G358 G359 G360 D362 D380 D387	G348 G349 G350 D352 D370 D377

Gene Ontology

Molecular Function

• GO:0004222 metalloendopeptidase activity
• GO:0005509 calcium ion binding
• GO:0008237 metallopeptidase activity
• GO:0008270 zinc ion binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006508 proteolysis

Cellular Component

• GO:0005615 extracellular space
• GO:0031012 extracellular matrix

External links

• PDB: RCSB:1o0q, PDBe:1o0q, PDBj:1o0q
• PDBsum: 1o0q
• PubMed: 12837794
• UniProt: O69771