Structure of PDB 1nno Chain A

Receptor sequence

>1nnoA (length=539) Species: 287 (Pseudomonas aeruginosa)

KAAEQYQGAASAVDPAHVVRTNGAPDMSESEFNEAKQIYFQRCAGCHGVL
RKGATGKPLTPDITQQRGQQYLEALITYGTPLGMPNWGSSGELSKEQITL
MAKYIQHTPPQPPEWGMPEMRESWKVLVKPEDRPKKQLNDLDLPNLFSVT
LRDAGQIALVDGDSKKIVKVIDTGYAVHISRMSASGRYLLVIGRDARIDM
IDLWAKEPTKVAEIKIGIEARSVESSKFKGYEDRYTIAGAYWPPQFAIMD
GETLEPKQIVSTRGMTVDTQTYHPEPRVAAIIASHEHPEFIVNVKETGKV
LLVNYKDIDNLTVTSIGAAPFLHDGGWDSSHRYFMTAANNSNKVAVIDSK
DRRLSALVDVGKTPHPGRGANFVHPKYGPVWSTSHLGDGSISLIGTDPKN
HPQYAWKKVAELQGQGGGSLFIKTHPKSSHLYVDTTFNPDARISQSVAVF
DLKNLDAKYQVLPIAEWADLGEGAKRVVQPEYNKRGDEVWFSVWNGKNDS
SALVVVDDKTLKLKAVVKDPRLITPTGKFNVYNTQHDVY

3D structure

• PDB: 1nno Conformational changes occurring upon reduction and NO binding in nitrite reductase from Pseudomonas aeruginosa.
• Chain: A
• Resolution: 2.65 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C47 C50 H51 M88 H327 H369
• Catalytic site (residue number reindexed from 1): C43 C46 H47 M84 H323 H365
• Enzyme Commision number: 1.7.2.1: nitrite reductase (NO-forming).
  1.7.99.1: hydroxylamine reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEC	A	C47 C50 H51 G60 K61 L63 R71 L79 T84 L86 M88 P89 W91	C43 C46 H47 G56 K57 L59 R67 L75 T80 L82 M84 P85 W87
BS02	DHE	A	R156 H182 I183 R198 R225 Y245 A283 H327 R372 F425 W498 F533	R152 H178 I179 R194 R221 Y241 A279 H323 R368 F421 W494 F529

Gene Ontology

Molecular Function

• GO:0009055 electron transfer activity
• GO:0016491 oxidoreductase activity
• GO:0020037 heme binding
• GO:0046872 metal ion binding
• GO:0050418 hydroxylamine reductase activity
• GO:0050421 nitrite reductase (NO-forming) activity

Cellular Component

• GO:0042597 periplasmic space

External links

• PDB: RCSB:1nno, PDBe:1nno, PDBj:1nno
• PDBsum: 1nno
• PubMed: 9760233
• UniProt: P24474|NIRS_PSEAE Nitrite reductase (Gene Name=nirS)