Structure of PDB 1nm9 Chain A

Receptor sequence

>1nm9A (length=496) Species: 9606 (Homo sapiens)

QYSSNTQQGRTSIVHLFEWRWVDIALECERYLAPKGFGGVQVSPPNENVA
IHNPFRPAWERYQPVSYKLCTRSGNEDEFRNMVTRCNNVGVRIYVDAVIN
HMCGNAVSAGTSSTCGSYFNPGSRDFPAVPYSGWDFNDGKCKTGSGDIEN
YNDATQVRDCRLSGLLDLALGKDYVRSKIAEYMNHLIDIGVAGFRIDASK
HMWPGDIKAILDKLHNLNSNWFPEGSKPFIYQEVIDLGGEPIKSSDYFGN
GRVTEFKYGAKLGTVIRKWNGEKMSYLKNWGEGWGFMPSDRALVFVDNHD
NQRGHGAGGASILTFWDARLYKMAVGFMLAHPYGFTRVMSSYRWPRYFEN
GKDVNDWVGPPNDNGVTKEVTINPDTTCGNDWVCEHRWRQIRNMVNFRNV
VDGQPFTNWYDNGSNQVAFGRGNRGFIVFNNDDWTFSLTLQTGLPAGTYC
DVISGDKINGNCTGIKIYVSDDGKAHFSISNSAEDPFIAIHAESKL

3D structure

• PDB: 1nm9 Human salivary alpha-amylase Trp58 situated at subsite -2 is critical for enzyme activity.
• Chain: A
• Resolution: 2.1 Å

Enzymatic activity

• Enzyme Commision number: 3.2.1.1: alpha-amylase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLC	A	K200 E240 G306	K200 E240 G306
BS02	AGL	A	H201 E233 D300	H201 E233 D300
BS03	GLC	A	E272 Y276 N279 W284	E272 Y276 N279 W284
BS04	AGL	A	K261 W284	K261 W284
BS05	HMC	A	Y62 H101 D197 E233 H299 D300	Y62 H101 D197 E233 H299 D300
BS06	CA	A	N100 R158 D167 H201	N100 R158 D167 H201

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004556 alpha-amylase activity
• GO:0005509 calcium ion binding
• GO:0016160 amylase activity
• GO:0016798 hydrolase activity, acting on glycosyl bonds
• GO:0031404 chloride ion binding
• GO:0043169 cation binding
• GO:0046872 metal ion binding

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0009311 oligosaccharide metabolic process

Cellular Component

• GO:0005576 extracellular region
• GO:0005615 extracellular space
• GO:0070062 extracellular exosome

External links

• PDB: RCSB:1nm9, PDBe:1nm9, PDBj:1nm9
• PDBsum: 1nm9
• PubMed: 15182367
• UniProt: P0DUB6|AMY1A_HUMAN Alpha-amylase 1A (Gene Name=AMY1A)