Structure of PDB 1nj6 Chain A

Receptor sequence
>1nj6A (length=463) Species: 187420 (Methanothermobacter thermautotrophicus str. Delta H) [Search protein sequence]
EFSEWFHNILEEAEIIDQRYPVKGMHVWMPHGFMIRKNTLKILRRILDRD
HEEVLFPLLVPEDELAKEAIHVKGFEDEVYWVTHGGLSKLQRKLALRPTS
ETVMYPMFALWVRSHTDLPMRFYQVVNTFRYETKHTRPLIRVREITTFKE
AHTIHATASEAEEQVERAVEIYKEFFNSLGIPYLITRRPPWDKFPGSEYT
VAFDTLMPDGKTLQIGTVHNLGQTFARTFEIKFETPEGDHEYVHQTCYGL
SDRVIASVIAIHGDESGLCLPPDVAAHQVVIVPIIFKKAAEEVMEACREL
RSRLEAAGFRVHLDDRDIRAGRKYYEWEMRGVPLRVEIGPRDLEKGAAVI
SRRDTGEKVTADLQGIEETLRELMKDILENLRTRAWERMESEIREAETLE
EASRIVDEKRGIISFMWCGEEECGMDVEEKVRVDILGIQEEGSGTCINCG
REAPYRAYLARTY
3D structure
PDB1nj6 The structural basis of cysteine aminoacylation of tRNAPro by prolyl-tRNA synthetases
ChainA
Resolution2.85 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) E119 R148 H170
Catalytic site (residue number reindexed from 1) E101 R130 H152
Enzyme Commision number 6.1.1.15: proline--tRNA ligase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ZN A C436 C441 C464 C418 C423 C446
BS02 MG A D210 E446 D452 D192 E428 D434
BS03 A5A A T117 E119 R148 I158 V160 I163 F212 Q232 G234 T235 H237 C265 Y266 G267 S269 R271 T99 E101 R130 I140 V142 I145 F194 Q214 G216 T217 H219 C247 Y248 G249 S251 R253
Gene Ontology
Molecular Function
GO:0000166 nucleotide binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004827 proline-tRNA ligase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0046872 metal ion binding
Biological Process
GO:0006412 translation
GO:0006418 tRNA aminoacylation for protein translation
GO:0006433 prolyl-tRNA aminoacylation
Cellular Component
GO:0005737 cytoplasm
GO:0017101 aminoacyl-tRNA synthetase multienzyme complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1nj6, PDBe:1nj6, PDBj:1nj6
PDBsum1nj6
PubMed12578991
UniProtO26708|SYP_METTH Proline--tRNA ligase (Gene Name=proS)

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