Structure of PDB 1n3e Chain A

Receptor sequence

>1n3eA (length=151) Species: 3055 (Chlamydomonas reinhardtii) [Search protein sequence]

TKYNKEFLLYLAGFVDGDGSIIAQIKPNQSYKFKHQLSLTFQVTQKTQRR
WFLDKLVDEIGVGYVRDRGSVSDYILSEIKPLHNFLTQLQPFLKLKQKQA
NLVLKIIEQLPSAKESPDKFLEVCTWVDQIAALNDSKTRKTTSETVRAVL
D

3D structure

PDB

1n3e Flexible DNA Target Site Recognition by Divergent Homing Endonuclease Isoschizomers I-CreI and I-MsoI

Chain

Resolution

2.5 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	G19 D20
Catalytic site (residue number reindexed from 1)	G17 D18
Enzyme Commision number	3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	dna	A	D20 T46 Q47 K48 R51	D18 T44 Q45 K46 R49
BS02	dna	A	G19 D20 G21 S22 I24 Q26 K28 Q44 R70 A133 N136 D137 S138 R141 K142	G17 D18 G19 S20 I22 Q24 K26 Q42 R68 A131 N134 D135 S136 R139 K140
BS03	dna	A	S32 Y33 K34 Q38 Y66 R68 R70 E80 I81 K116 K139	S30 Y31 K32 Q36 Y64 R66 R68 E78 I79 K114 K137

Gene Ontology

	Molecular Function
GO:0004519	endonuclease activity
GO:0042802	identical protein binding
GO:0046872	metal ion binding

	Biological Process
GO:0006314	intron homing

	Cellular Component
GO:0009507	chloroplast

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:1n3e, PDBe:1n3e, PDBj:1n3e
PDBsum	1n3e
PubMed	12758074
UniProt	P05725\|DNE1_CHLRE DNA endonuclease I-CreI

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