Structure of PDB 1n1h Chain A

Receptor sequence

>1n1hA (length=1264) Species: 10886 (Mammalian orthoreovirus 3 Dearing)

SSMILTQFGPFIESISGITDQSNDVFEDAAKAFSMFTRSDVYKALDEIPF
SDDAMLPIPPTIYTKPSHDSYYYIDALNRVRRKTYQGPDDVYVPNCSIVE
LLEPHETLTSYGRLSEAIENRAKDGDSQARIATTYGRIAESQARQIKAPL
EKFVLALLVAEAGGSLYDPVLQKYDEIPDLSHNCPLWCFREICRHISGPL
PDRAPYLYLSAGVFWLMSPRMTSAIPPLLSDLVNLAILQQTAGLDPSLVK
LGVQICLHAAASSSYSWFILKTKSIFPQNTLHSMYESLEGGYCPNLEWLE
PRSDYKFMYMGVMPLSAKYARSAPSNDKKARELGEKYGLSSVVGELRKRT
KTYVKHDFASVRYIRDAMACTSGIFLVRTPTETVLQEYTQSPEIKVPIPQ
KDWTGPIGEIRILKDTTSSIARYLYRTWYLAAARMAAQPRTWDPLFQAIM
RSQYVTARGGSGAALRESLYAINVSLPDFKGLPVKAATKIFQAAQLANLP
FSHTSVAILADTSMGLRNQVQRRPRSIMPLNVPQQQVSAPHTLTADYINY
HMNLSPTSGSAVIEKVIPLGVYASSPPNQSINIDISACDASITWDFFLSV
IMAAIHEGVASSSIGKPFMGVPASIVNDESVVGVRAARPISGMQNMIQHL
SKLYKRGFSYRVNDSFSPGNDFTHMTTTFPSGSTATSTEHTANNSTMMET
FLTVWGPEHTDDPDVLRLMKSLTIQRNYVCQGDDGLMIIDGTTAGKVNSE
TIQNDLELISKYGEEFGWKYDIAYDGTAEYLKLYFIFGCRIPNLSRHPIV
GKERANSSAEEPWPAILDQIMGVFFNGVHDGLQWQRWIRYSWALCCAFSR
QRTMIGESVGYLQYPMWSFVYWGLPLVKAFGSDPWIFSWYMPTGDLGMYS
WISLIRPLMTRWMVANGYVTDRCSTVFGNADYRRCFNELKLYQGYYMAQL
PRNPKKSGRAASREVREQFTQALSDYLMQNPELKSRVLRGRSEWEKYGAG
IIHNPPSLFDVPHKWYQGAQEAAIATREELAEMDETLMRARRHSYSSFSK
LLEAYLLVKWRMCEAREPSVDLRLPLCAGIDPLNSDPFLKMVSVGPMLQS
TRKYFAQTLFMAKTVSGLDVNAIDSALLRLRTLGADKKALTAQLLMVGLQ
ESEADALAGKIMLQDVNTVQLARVVNLAVPDTWMSLDFDSMFKHHVKLLP
KDGRHLNTDIPPRMGWLRAILRFLGAGMVMTATGVAVDIYLEDIHGGGRS
LGQRFMTWMRQEGR

3D structure

• PDB: 1n1h RNA Synthesis in a Cage--Structural Studies of Reovirus Polymerase [lambda] 3
• Chain: A
• Resolution: 2.8 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	T457 R459 G460 S462 K490 S514 M515 R518 I528 P530 S682 G683 N807	T456 R458 G459 S461 K489 S513 M514 R517 I527 P529 S681 G682 N806
BS02	MN	A	D585 I586 D734	D584 I585 D733
BS03	MN	A	D585 D734 D735	D584 D733 D734
BS04	G7M	A	S211 P813 W814 Q852 R853 D1035	S210 P812 W813 Q851 R852 D1034
BS05	GDP	A	K32 S35 M36 R851 Q852 Y862	K31 S34 M35 R850 Q851 Y861
BS06	CH1	A	R523 R524 R526 S587 D590 S682 D734	R522 R523 R525 S586 D589 S681 D733
BS07	GH3	A	S561 K566 R797 H798	S560 K565 R796 H797

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0016787 hydrolase activity
• GO:0034062 5'-3' RNA polymerase activity

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0019079 viral genome replication
• GO:0032774 RNA biosynthetic process

Cellular Component

• GO:0019013 viral nucleocapsid
• GO:0019028 viral capsid

External links

• PDB: RCSB:1n1h, PDBe:1n1h, PDBj:1n1h
• PDBsum: 1n1h
• PubMed: 12464184
• UniProt: P0CK31|RDRP_REOVD RNA-directed RNA polymerase lambda-3 (Gene Name=L1)