Structure of PDB 1mxt Chain A

Receptor sequence

>1mxtA (length=498) Species: 1931 (Streptomyces sp.)

GYVPAVVIGTGYGAAVSALRLGEAGVQTLMLEMGQLWNQPGPDGNIFCGM
LNPDKRSSWFKNRTEAPLGSFLWLDVVNRNIDPYAGVLDRVNYDQMSVYV
GRGVGGGSLVNGGMAVEPKRSYFEEILPRVDSSEMYDRYFPRANSMLRVN
HIDTKWFEDTEWYKFARVSREQAGKAGLGTVFVPNVYDFGYMQREAAGEV
PKSALATEVIYGNNHGKQSLDKTYLAAALGTGKVTIQTLHQVKTIRQTKD
GGYALTVEQKDTDGKLLATKEISCRYLFLGAGSLGSTELLVRARDTGTLP
NLNSEVGAGWGPNGNIMTARANHMWNPTGAHQSSIPALGIDAWDNSDSSV
FAEIAPMPAGLETWVSLYLAITKNPQRGTFVYDAATDRAKLNWTRDQNAP
AVNAAKALFDRINKANGTIYRYDLFGTQLKAFADDFCYHPLGGCVLGKAT
DDYGRVAGYKNLYVTDGSLIPGSVGVNPFVTITALAERNVERIIKQDV

3D structure

• PDB: 1mxt Sub-atomic resolution crystal structure of cholesterol oxidase: What atomic resolution crystallography reveals about enzyme mechanism and the role of FAD cofactor in redox activity
• Chain: A
• Resolution: 0.95 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): E361 H447 N485
• Catalytic site (residue number reindexed from 1): E353 H439 N477
• Enzyme Commision number: 1.1.3.6: cholesterol oxidase.
  5.3.3.1: steroid Delta-isomerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAE	A	I16 G17 G19 Y20 L39 E40 M41 Y107 R110 G114 G115 N119 G120 M122 Q249 V250 A289 G290 Y446 H447 D474 G475 N485 P486 F487	I8 G9 G11 Y12 L31 E32 M33 Y99 R102 G106 G107 N111 G112 M114 Q241 V242 A281 G282 Y438 H439 D466 G467 N477 P478 F479
BS02	OXY	A	M122 E361 N485	M114 E353 N477

Gene Ontology

Molecular Function

• GO:0004769 steroid delta-isomerase activity
• GO:0016491 oxidoreductase activity
• GO:0016614 oxidoreductase activity, acting on CH-OH group of donors
• GO:0016853 isomerase activity
• GO:0016995 cholesterol oxidase activity
• GO:0050660 flavin adenine dinucleotide binding

Biological Process

• GO:0006707 cholesterol catabolic process
• GO:0008203 cholesterol metabolic process

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:1mxt, PDBe:1mxt, PDBj:1mxt
• PDBsum: 1mxt
• PubMed: 12595270
• UniProt: P12676|CHOD_STRS0 Cholesterol oxidase (Gene Name=choA)