Structure of PDB 1mwh Chain A

Receptor sequence

>1mwhA (length=1256) Species: 10891 (Reovirus sp.)

SSMILTQFGPFIESISGITDQSNDVFEDAAKAFSMFTRSDVYKALDEIPF
SDDAMLPIPPTIYTKPSHDSYYYIDALNRVRRKTYQGPDDVYVPNCSIVE
LLEPHETLTSYGRLSEAIENRAKDGDSQARIATTYGRIAESQARQIKAPL
EKFVLALLVAEAGGSLYDPVLQKYDEIPDLSHNCPLWCFREICRHISGPL
PDRAPYLYLSAGVFWLMSPRMTSAIPPLLSDLVNLAILQQTAGLDPSLVK
LGVQICLHAAASSSYSWFILKTKSIFPQNTLHSMYESLEGGYCPNLEWLE
PRSDYKFMYMGVMPLSAKYARSAPSNDKKARELGEKYGLSSVVGELRKRT
KTYVKHDFASVRYIRDAMACTSGIFLVRTPTETVLQEYTQSPEIKVPIPQ
KDWTGPIGEIRILKDTTSSIARYLYRTWYLAAARMAAQPRTWDPLFQAIM
RSQYVTARGGSGAALRESLYAINVSLPDFKGLPVKAATKIFQAAQLANLP
FSHTSVAILADTSMGLRNQVQRRPRSIMPLNVPQQQVSAPHTLTADYINY
HMNLSPTSGSAVIEKVIPLGVYASSPPNQSINIDISACDASITWDFFLSV
IMAAIHEGVASSSIGKPFMGVPASIVNDESVVGVRAARPISGMQNMIQHL
SKLYKRGFSYRVNDSFSPGNDFTHMTTTFPSGSTATSTEHTANNSTMMET
FLTVWGPEHTDDPDVLRLMKSLTIQRNYVCQGDDGLMIIDGTTAGKVNSE
TIQNDLELISKYGEEFGWKYDIAYDGTAEYLKLYFIFGCRIPNLSRHPIV
GKERANSSAEEPWPAILDQIMGVFFNGVHDGLQWQRWIRYSWALCCAFSR
QRTMIGESVGYLQYPMWSFVYWGLPLVKAFGSDPWIFSWYMPTGDLGMYS
WISLIRPLMTRWMVANGYVTDRCSTVFGNADYRRCFNELKLYQGYYMAQL
PRNPKEVREQFTQALSDYLMQNPELKSRVLRGRSEWEKYGAGIIHNPPSL
FDVPHKWYQGAQEAAIATREELAEMDETLMRARRHSYSSFSKLLEAYLLV
KWRMCEAREPSVDLRLPLCAGIDPLNSDPFLKMVSVGPMLQSTRKYFAQT
LFMAKTVSGLDVNAIDSALLRLRTLGADKKALTAQLLMVGLQESEADALA
GKIMLQDVNTVQLARVVNLAVPDTWMSLDFDSMFKHHVKLLPKDGRHLNT
DIPPRMGWLRAILRFLGAGMVMTATGVAVDIYLEDIHGGGRSLGQRFMTW
MRQEGR

3D structure

• PDB: 1mwh RNA Synthesis in a Cage-Structural Studies of Reovirus Polymerase lambda3
• Chain: A
• Resolution: 2.5 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MN	A	D585 D735 E780	D584 D734 E779
BS02	GTG	A	S35 M36 W814 R851 Q852 R853 Y862 D1035	S34 M35 W813 R850 Q851 R852 Y861 D1026

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0016787 hydrolase activity
• GO:0034062 5'-3' RNA polymerase activity

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0019079 viral genome replication
• GO:0032774 RNA biosynthetic process

Cellular Component

• GO:0019013 viral nucleocapsid
• GO:0019028 viral capsid

External links

• PDB: RCSB:1mwh, PDBe:1mwh, PDBj:1mwh
• PDBsum: 1mwh
• PubMed: 12464184
• UniProt: P0CK31|RDRP_REOVD RNA-directed RNA polymerase lambda-3 (Gene Name=L1)