Structure of PDB 1mwe Chain A

Receptor sequence

>1mweA (length=388) Species: 11320 (Influenza A virus)

RDFNNLTKGLCTINSWHIYGKDNAVRIGEDSDVLVTREPYVSCDPDECRF
YALSQGTTIRGKHSNGTIHDRSQYRALISWPLSSPPTVYNSRVECIGWSS
TSCHDGKTRMSICISGPNNNASAVIWYNRRPVTEINTWARNILRTQESEC
VCHNGVCPVVFTDGSATGPAETRIYYFKEGKILKWEPLAGTAKHIEECSC
YGERAEITCTCRDNWQGSNRPVIRIDPVAMTHTSQYICSPVLTDNPRPND
PTVGKCNDPYPGNNNNGVKGFSYLDGVNTWLGRTISIASRSGYEMLKVPN
ALTDDKSKPTQGQTIVLNTDWSGYSGSFMDYWAEGECYRACFYVELIRGR
PKEDKVWWTSNSIVSMCSSTEFLGQWDWPDGAKIEYFL

3D structure

• PDB: 1mwe Structural evidence for a second sialic acid binding site in avian influenza virus neuraminidases.
• Chain: A
• Resolution: 1.7 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D151 E277 R292 R371 Y406
• Catalytic site (residue number reindexed from 1): D70 E197 R212 R290 Y324
• Enzyme Commision number: 3.2.1.18: exo-alpha-sialidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SIA	A	R118 E119 D151 R152 W178 R224 A246 E276 R292 R371 Y406	R37 E38 D70 R71 W98 R144 A166 E196 R212 R290 Y324
BS02	SIA	A	S367 S370 S372 N400 T401 D402 W403 K432	S286 S289 S291 N318 T319 D320 W321 K352
BS03	CA	A	D293 N294 G297 D324 N347	D213 N214 G217 D244 N266

Gene Ontology

Molecular Function

• GO:0004308 exo-alpha-sialidase activity

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0046761 viral budding from plasma membrane

Cellular Component

• GO:0016020 membrane
• GO:0033644 host cell membrane
• GO:0055036 virion membrane

External links

• PDB: RCSB:1mwe, PDBe:1mwe, PDBj:1mwe
• PDBsum: 1mwe
• PubMed: 9342319
• UniProt: P03472|NRAM_I75A5 Neuraminidase (Gene Name=NA)