Structure of PDB 1mus Chain A

Receptor sequence

>1musA (length=458) Species: 562 (Escherichia coli)

SALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKSITISSEGS
KAAQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSY
RHQVAEELGKLGSIQKASRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDD
PADADEKESGKWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAH
NERFVVRSKHPRKDVESGLYLYDHLKNQPELGGYQISIPQKGVVDKRGKR
KNRPARKASLSLRSGRITLKQGNITLNAVLAEEINPPKGETPLKWLLLTS
EPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAERQRMEKPDNLERMV
SILSFVAVRLLQLRESFTPPSQSAETVLTPDECQLLGYLDKGKRKRKEKA
GSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQSKLDGFLAAKDL
MAQGIKIG

3D structure

• PDB: 1mus Structure/function insights into Tn5 transposition.
• Chain: A
• Resolution: 1.9 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	T99 Q243 K244 G245 N255 W323 E326 H329 K330 K333 T334 K439	T96 Q240 K241 G242 N252 W320 E323 H326 K327 K330 T331 K420
BS02	dna	A	D97 D188 R210 Y237 K244 L296 W298 Y319 R322 W323 R367 F435 S438 K439 I443 S445	D94 D185 R207 Y234 K241 L293 W295 Y316 R319 W320 R364 F416 S419 K420 I424 S426
BS03	MN	A	D97 E326	D94 E323
BS04	MN	A	D97 D188	D94 D185
BS05	MG	A	S178 S181	S175 S178
BS06	MG	A	E451 E454	E432 E435

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0004519 endonuclease activity
• GO:0004803 transposase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006310 DNA recombination
• GO:0006313 DNA transposition
• GO:0032196 transposition

External links

• PDB: RCSB:1mus, PDBe:1mus, PDBj:1mus
• PDBsum: 1mus
• PubMed: 15102449
• UniProt: Q46731|TN5P_ECOLX Transposase for transposon Tn5 (Gene Name=tnpA)