Structure of PDB 1muh Chain A

Receptor sequence

>1muhA (length=455) Species: 562 (Escherichia coli)

ALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKSITISSEGSK
AAQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR
HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDP
ADADEKESGKWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHN
ERFVVRSKHPRKDVESGLYLYDHLKNQPELGGYQISIPQKGVVDKRGKRK
NRPARKASLSLRSGRITLKQGNITLNAVLAEEINPPKGETPLKWLLLTSE
PVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAERQRMEEPDNLERMVS
ILSFVAVRLLQLRESFTPQSAETVLTPDECQLLGYLDKGKRKRKEKAGSL
QWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQSKLDGFLAAKDLMAQ
GIKIG

3D structure

• PDB: 1muh Three-dimensional structure of the Tn5 synaptic complex transposition intermediate.
• Chain: A
• Resolution: 2.3 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	T99 Q243 K244 G245 N255 W323 E326 H329 K330 K333 T334 K439 R440	T95 Q239 K240 G241 N251 W319 E322 H325 K326 K329 T330 K417 R418
BS02	dna	A	D188 R210 Y237 I239 W298 Y319 R322 W323 R367 S438 K439 I443 S445	D184 R206 Y233 I235 W294 Y315 R318 W319 R363 S416 K417 I421 S423
BS03	MN	A	D97 E326	D93 E322
BS04	MG	A	E110 E345	E106 E341

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0004519 endonuclease activity
• GO:0004803 transposase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006310 DNA recombination
• GO:0006313 DNA transposition
• GO:0032196 transposition

External links

• PDB: RCSB:1muh, PDBe:1muh, PDBj:1muh
• PDBsum: 1muh
• PubMed: 10884228
• UniProt: Q46731|TN5P_ECOLX Transposase for transposon Tn5 (Gene Name=tnpA)