Structure of PDB 1mio Chain A

Receptor sequence

>1mioA (length=525) Species: 1501 (Clostridium pasteurianum)

SENLKDEILEKYIPKTKKTRSGHIVIKTEETPNPEIVANTRTVPGIITAR
GCAYAGCKGVVMGPIKDMVHITHGPIGCSFYTWGGRRFKSKPENGTGLNF
NEYVFSTDMQESDIVFGGVNKLKDAIHEAYEMFHPAAIGVYATCPVGLIG
DDILAVAATASKEIGIPVHAFSCEGYKGVSQSAGHHIANNTVMTDIIGKG
NKEQKKYSINVLGEYNIGGDAWEMDRVLEKIGYHVNATLTGDATYEKVQN
ADKADLNLVQCHRSINYIAEMMETKYGIPWIKCNFIGVDGIVETLRDMAK
CFDDPELTKRTEEVIAEEIAAIQDDLDYFKEKLQGKTACLYVGGSRSHTY
MNMLKSFGVDSLVAGFEFAHRDDYEGREVIPTIKIDADSKNIPEITVTPD
EQKYRVVIPEDKVEELKKAGVPLSSYGGMMKEMHDGTILIDDMNHHDMEV
VLEKLKPDMFFAGIKEKFVIQKGGVLSKQLHSYDYNGPYAGFRGVVNFGH
ELVNGIYTPAWKMITPPWKKASSES

3D structure

• PDB: 1mio X-ray crystal structure of the nitrogenase molybdenum-iron protein from Clostridium pasteurianum at 3.0-A resolution.
• Chain: A
• Resolution: 3.0 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C53 A56 R87 H186
• Catalytic site (residue number reindexed from 1): C52 A55 R86 H185
• Enzyme Commision number: 1.18.6.1: nitrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CFM	A	V61 R87 H186 Y216 C262 S265 G344 G345 S346 R347 F369 H482	V60 R86 H185 Y215 C261 S264 G343 G344 S345 R346 F368 H481
BS02	CLP	A	C53 Y55 P76 G78 C79 C145 G176	C52 Y54 P75 G77 C78 C144 G175

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0016163 nitrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0051536 iron-sulfur cluster binding

Biological Process

• GO:0009399 nitrogen fixation

Cellular Component

• GO:0016612 molybdenum-iron nitrogenase complex

External links

• PDB: RCSB:1mio, PDBe:1mio, PDBj:1mio
• PDBsum: 1mio
• PubMed: 8393705
• UniProt: P00467|NIFD_CLOPA Nitrogenase molybdenum-iron protein alpha chain (Gene Name=nifD)