Structure of PDB 1mdl Chain A

Receptor sequence
>1mdlA (length=357) Species: 287 (Pseudomonas aeruginosa) [Search protein sequence]
EVLITGLRTRAVNVPLAYPVHTAVGTVGTAPLVLIDLATSAGVVGHSYLF
AYTPVALKSLKQLLDDMAAMIVNEPLAPVSLEAMLAKRFCLAGYTGLIRM
AAAGIDMAAWDALGKVHETPLVKLLGANARPVQAYDSHSLDGVKLATERA
VTAAELGFRAVKTRIGYPALDQDLAVVRSIRQAVGDDFGIMVDYNQSLDV
PAAIKRSQALQQEGVTWIEEPTLQHDYEGHQRIQSKLNVPVQMGENWLGP
EEMFKALSIGACRLAMPDAMKIGGVTGWIRASALAQQFGIPMSSHLFQEI
SAHLLAATPTAHWLERLDLAGSVIEPTLTFEGGNAVIPDLPGVGIIWREK
EIGKYLV
3D structure
PDB1mdl Mechanism of the reaction catalyzed by mandelate racemase: structure and mechanistic properties of the K166R mutant.
ChainA
Resolution1.85 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) V22 S139 K164 R166 D195 N197 E221 G246 E247 D270 M294 H297 L298 F299 A313 E317
Catalytic site (residue number reindexed from 1) V20 S137 K162 R164 D193 N195 E219 G244 E245 D268 M292 H295 L296 F297 A311 E315
Enzyme Commision number 5.1.2.2: mandelate racemase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG A D195 E221 E247 D193 E219 E245
BS02 RMN A V22 F52 R166 V20 F50 R164 PDBbind-CN: -logKd/Ki=3.09,Ki=810uM
BindingDB: Ki=810000nM
BS03 SMN A K164 R166 D195 E247 H297 E317 K162 R164 D193 E245 H295 E315 BindingDB: Ki=570000nM
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0016836 hydro-lyase activity
GO:0016853 isomerase activity
GO:0018838 mandelate racemase activity
GO:0046872 metal ion binding
Biological Process
GO:0009063 amino acid catabolic process
GO:0016052 carbohydrate catabolic process
GO:0018924 mandelate metabolic process
GO:0019596 mandelate catabolic process

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Molecular Function
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Biological Process
External links
PDB RCSB:1mdl, PDBe:1mdl, PDBj:1mdl
PDBsum1mdl
PubMed7893690
UniProtP11444|MANR_PSEPU Mandelate racemase (Gene Name=mdlA)

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