Structure of PDB 1m0w Chain A

Receptor sequence

>1m0wA (length=481) Species: 4932 (Saccharomyces cerevisiae)

PSKDQLNELIQEVNQWAITNGLSMYPPKFEENPSNASVSPVTIYPTPIPR
KCFDEAVQIQPVFNELYARITQDMAQPDSYLHKTTEALALSDSEFTGKLW
SLYLATLKSAQYKKQNFRLGIFRSDYLIDKKKGTEQIKQVEFNTVSVSFA
GLSEKVDRLHSYLNRANKYDPKGPIYNDQNMVISDSGYLLSKALAKAVES
YKSQQSDPIVAFIVQRNERNVFDQKVLELNLLEKFGTKSVRLTFDDVNDK
LFIDDKTGKLFIRDTEQEIAVVYYRTGYTTTDYTSEKDWEARLFLEKSFA
IKAPDLLTQLSGSKKIQQLLTDEGVLGKYISDAEKKSSLLKTFVKIYPLD
DTKLGREGKRLALSEPSKYVLKPQREGGGNNVYKENIPNFLKGIEERHWD
AYILMELIEPELNENNIILRDNKSYNEPIISELGIYGCVLFNDEQVLSNE
FSGSLLRSKFNTSNEGGVAAGFGCLDSIILY

3D structure

• PDB: 1m0w Large Conformational Changes in the Catalytic Cycle of Glutathione Synthase
• Chain: A
• Resolution: 1.8 Å

Enzymatic activity

• Enzyme Commision number: 6.3.2.3: glutathione synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MG	A	E146 E386	E141 E376
BS02	ANP	A	L132 E146 K324 K382 E386 G387 N391 Y393 M415 L417 I418 E442 K469	L127 E141 K314 K372 E376 G377 N381 Y383 M405 L407 I408 E432 K459
BS03	3GC	A	R128 N148 S151 V152 S153 E228 Q234 R285 Y288	R123 N143 S146 V147 S148 E218 Q224 R275 Y278

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0004363 glutathione synthase activity
• GO:0005524 ATP binding
• GO:0016874 ligase activity
• GO:0042803 protein homodimerization activity
• GO:0043295 glutathione binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006750 glutathione biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:1m0w, PDBe:1m0w, PDBj:1m0w
• PDBsum: 1m0w
• PubMed: 12467574
• UniProt: Q08220|GSHB_YEAST Glutathione synthetase GSH2 (Gene Name=GSH2)