Structure of PDB 1lwt Chain A

Receptor sequence

>1lwtA (length=448) Species: 4932 (Saccharomyces cerevisiae)

CFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPREVIKLPRGRETMYSV
VQKSQHRAHKSSREVPELLKFTCNATHELVVRTPRSVRRLSRTIKGVEYF
EVITFEMGQKKAPDGRIVELVKEVSKSYPISEGPERANELVESYRKASNK
AYFEWTIEARDLSLLGSHVRKATYQTYAPILYENDHFFDYMQKSKFHLTI
EGPKVLAYLLGLWIGDGLSDRATFSVDSRDTSLMERVTEYAEKLNLCAEY
KDRKAKTVNLYSKVVRGNGIRNNLNTENPLWDAIVGLGFLKDGVKNIPSF
LSTDNIGTRETFLAGLIDSDGYVTDEHGIKATIKTIHTSVRDGLVSLARS
LGLVVSVNAEPAKVDMNGTKHKISYAIYMSGGDVLLNVLSKCAGSKKFRP
APAAAFARECRGFYFELQELKEDDYYGITLSDDSDHQFLLANQVVVHN

3D structure

• PDB: 1lwt Crystal structure of the intein homing endonuclease PI-SceI bound to its recognition sequence.
• Chain: A
• Resolution: 3.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C1 N76 T78 H79 K301 K403 G433 I434 N454
• Catalytic site (residue number reindexed from 1): C1 N74 T76 H77 K295 K397 G427 I428 N448
• Enzyme Commision number: 3.1.-.-
  7.1.2.2: H(+)-transporting two-sector ATPase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	K53 Q55 R57 R90 L92 R94 K173 A174 R223 Y267 R277 N278 N279 L280 N281 T282 Y328 K340 T375 K376 H377 K378 Y420	K53 Q55 R57 R88 L90 R92 K171 A172 R221 Y261 R271 N272 N273 L274 N275 T276 Y322 K334 T369 K370 H371 K372 Y414
BS02	dna	A	Q55 H56 R57 V126 S127 K128 S129 G168 S169 H170 S227 A261 N274 R277 K336 S362 N364 E366 H377 Y384	Q55 H56 R57 V124 S125 K126 S127 G166 S167 H168 S225 A255 N268 R271 K330 S356 N358 E360 H371 Y378

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0004519 endonuclease activity

Biological Process

• GO:0016539 intein-mediated protein splicing
• GO:0030908 protein splicing

External links

• PDB: RCSB:1lwt, PDBe:1lwt, PDBj:1lwt
• PDBsum: 1lwt
• PubMed: 12219083
• UniProt: P17255|VATA_YEAST V-type proton ATPase catalytic subunit A (Gene Name=VMA1)