Structure of PDB 1lws Chain A

Receptor sequence

>1lwsA (length=427) Species: 4932 (Saccharomyces cerevisiae)

CFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPREVIKLPRGRETMYSV
VQKSQHRAHKSDSSREVPELLKFTCNATHELVVRTPRSVRRLSRTIKGVF
EVITFEMGQKKAPDGRIVELVKEVSKSYPISPERANELVESYRKASNKAY
FEWTIEARDLSLLGSHVRKATYQTYAPILYENDHFFDYMQKSKFHLTIEG
PKVLAYLLGLWIGDGLSDRATFSVDSRDTSLMERVTEYAEKLNLCAVAKT
VNLYSENPLWDAIVGLGFLKDGVKNIPSFLSTDNIGTRETFLAGLIDSDG
YVTDEHGIKATIKTIHTSVRDGLVSLARSLGLVVSVNAEPAKVDMNGTKH
KISYAIYMSGGDVLLNVLSKCAGSKKFRPAPAAAFARECRGFYFELQELK
EDDYYGITLSDDSDHQFLLANQVVVHN

3D structure

• PDB: 1lws Crystal structure of the intein homing endonuclease PI-SceI bound to its recognition sequence.
• Chain: A
• Resolution: 3.5 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C1 N76 T78 H79 K301 K403 G433 I434 N454
• Catalytic site (residue number reindexed from 1): C1 N76 T78 H79 K274 K376 G406 I407 N427
• Enzyme Commision number: 3.1.-.-
  7.1.2.2: H(+)-transporting two-sector ATPase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	K53 Q55 R57 R90 R94 A174 Y267 Y328 K340 T341 I342 T375 K376 H377 K378 Y420	K53 Q55 R57 R90 R94 A170 Y254 Y301 K313 T314 I315 T348 K349 H350 K351 Y393
BS02	dna	A	Q55 H56 R57 A58 R65 K124 S127 K128 S129 S169 H170 S227 V260 A261 H333 S362 N364 E366 N373 H377 Y384	Q55 H56 R57 A58 R65 K122 S125 K126 S127 S165 H166 S223 V247 A248 H306 S335 N337 E339 N346 H350 Y357
BS03	CA	A	G217 D218 D326	G213 D214 D299
BS04	CA	A	D218 S325 D326	D214 S298 D299

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0004519 endonuclease activity

Biological Process

• GO:0016539 intein-mediated protein splicing
• GO:0030908 protein splicing

External links

• PDB: RCSB:1lws, PDBe:1lws, PDBj:1lws
• PDBsum: 1lws
• PubMed: 12219083
• UniProt: P17255|VATA_YEAST V-type proton ATPase catalytic subunit A (Gene Name=VMA1)