Structure of PDB 1ltd Chain A

Receptor sequence

>1ltdA (length=481) Species: 4932 (Saccharomyces cerevisiae)

KISPAEVAKHNKPDDCWVVINGYVYDLTRFLPNHPGGQDVIKFNAGKDVT
AIFEPLHAPNVIDKYIAPEKKLGPLQGSMPPELVCPPYAPGETKEDIARK
EQLKSLLPPLDNIINLYDFEYLASQTLTKQAWAYYSSGANDEVTHRENHN
AYHRIFFKPKILVDVRKVDISTDMLGSHVDVPFYVSATALCKLGNPLEGE
KDVARGCGQGVTKVPQMISTLASCSPEEIIEAAPSDKQIQWYQLYVNSDR
KITDDLVKNVEKLGVKALFVTVDAPSLGQREKDMKLKFSNALSKFIDPSL
TWKDIEELKKKTKLPIVIKGVQRTEDVIKAAEIGVSGVVLSNHGGRQLDF
SRAPIEVLAETMPILEQRNLKDKLEVFVDGGVRRGTDVLKALCLGAKGVG
LGRPFLYANSCYGRNGVEKAIEILRDEIEMSMRLLGVTSIAELKPDLLDL
STLKARTVGVPNDVLYNEVYEGPTLTEFEDA

3D structure

• PDB: 1ltd The 2.6-A refined structure of the Escherichia coli recombinant Saccharomyces cerevisiae flavocytochrome b2-sulfite complex.
• Chain: A
• Resolution: 2.6 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): S228 Y254 T280 D282 K349 H373
• Catalytic site (residue number reindexed from 1): S219 Y245 T271 D273 K319 H343
• Enzyme Commision number: 1.1.2.3: L-lactate dehydrogenase (cytochrome).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SO3	A	H373 R376	H343 R346
BS02	FMN	A	Y144 S195 A196 T197 A198 S228 Q252 Y254 K349 H373 G374 R376 D409 G410 R413 G432 R433	Y135 S186 A187 T188 A189 S219 Q243 Y245 K319 H343 G344 R346 D379 G380 R383 G402 R403
BS03	HEM	A	F39 H43 P44 V49 I50 V58 I61 F62 H66 I71 I75 Y97 Q139 Y143 L199 L230	F30 H34 P35 V40 I41 V49 I52 F53 H57 I62 I66 Y88 Q130 Y134 L190 L221

Gene Ontology

Molecular Function

• GO:0016491 oxidoreductase activity
• GO:0020037 heme binding

External links

• PDB: RCSB:1ltd, PDBe:1ltd, PDBj:1ltd
• PDBsum: 1ltd
• PubMed: 8003966
• UniProt: P00175|CYB2_YEAST L-lactate dehydrogenase (cytochrome) (Gene Name=CYB2)