Structure of PDB 1lpq Chain A

Receptor sequence

>1lpqA (length=557) Species: 9606 (Homo sapiens)

KWKWWEEERYPEGIKWKFLEHKGPVFAPPYEPLPENVKFYYDGKVMKLSP
KAEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNEEKNIITNLSKCD
FTQMSQYFKAQTEARKQMSKEEKLKIKEENEKLLKEYGFCIMDNHKERIA
NFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHKW
KEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLK
KCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADT
VGCCSLRVEHINLHPELDGQEYVVEFDFLGKDSIRYYNKVPVEKRVFKNL
QLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQ
LKELTAPDENIPAKILSYNRANRAVAILCNHQEKSMMNLQTKIDAKKEQL
ADARRDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQLMKLEVQATDR
EENKQIALGTSKLNFLDPRITVAWCKKWGVPIEKIYNKTQREKFAWAIDM
ADEDYEF

3D structure

• PDB: 1lpq 8-Oxoguanine rearranges the active site of human topoisomerase I
• Chain: A
• Resolution: 3.14 Å

Enzymatic activity

• Enzyme Commision number: 5.6.2.1: DNA topoisomerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	R364 V410 T411 W412 Y426 K436 K439 K443 K587 H632 Q633 T718 N722	R163 V209 T210 W211 Y225 K235 K238 K242 K386 H431 Q432 T510 N514
BS02	dna	A	E356 G363 H367 K425 R488 G490 N491 K493 T501 K532 D533 Q578 T585 A586 K587 N745 K746 T747	E155 G162 H166 K224 R287 G289 N290 K292 T300 K331 D332 Q377 T384 A385 K386 N537 K538 T539

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003917 DNA topoisomerase type I (single strand cut, ATP-independent) activity

Biological Process

• GO:0006265 DNA topological change

Cellular Component

• GO:0005694 chromosome

External links

• PDB: RCSB:1lpq, PDBe:1lpq, PDBj:1lpq
• PDBsum: 1lpq
• PubMed: 12209008
• UniProt: P11387|TOP1_HUMAN DNA topoisomerase 1 (Gene Name=TOP1)