Structure of PDB 1lco Chain A

Receptor sequence

>1lcoA (length=480) Species: 4932 (Saccharomyces cerevisiae)

KISPAEVAKHNKPDDCWVVINGYVYDLTRFLPNHPGGQDVIKFNAGKDVT
AIFEPLHAPNVIDKYIAPEKKLGPLQGSMPPELVCPPYAPGETKEDIARK
EQLKSLLPPLDNIINLYDFEYLASQTLTKQAWAFYSSGANDEVTHRENHN
AYHRIFFKPKILVDVRKVDISTDMLGSHVDVPFYVSATALCKLGNPLEGE
KDVARGCGQGVTKVPQMISTLASCSPEEIIEAAPSDKQIQWYQLYVNSDR
KITDDLVKNVEKLGVKALFVTVDAPSLGQREKDMKLKFSALSKFIDPSLT
WKDIEELKKKTKLPIVIKGVQRTEDVIKAAEIGVSGVVLSNHGGRQLDFS
RAPIEVLAETMPILEQRNLKDKLEVFVDGGVRRGTDVLKALCLGAKGVGL
GRPFLYANSCYGRNGVEKAIEILRDEIEMSMRLLGVTSIAELKPDLLDLS
TLKARTVGVPNDVLYNEVYEGPTLTEFEDA

3D structure

• PDB: 1lco X-ray structure of two complexes of the Y143F flavocytochrome b2 mutant crystallized in the presence of lactate or phenyl lactate.
• Chain: A
• Resolution: 2.9 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): S228 Y254 T280 D282 K349 H373
• Catalytic site (residue number reindexed from 1): S219 Y245 T271 D273 K318 H342
• Enzyme Commision number: 1.1.2.3: L-lactate dehydrogenase (cytochrome).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEM	A	F39 H43 P44 V49 I50 V58 I61 F62 L65 H66 I75 Y97 Q139 F143 L199 L230 K296	F30 H34 P35 V40 I41 V49 I52 F53 L56 H57 I66 Y88 Q130 F134 L190 L221 K287
BS02	FMN	A	Y144 S195 A196 T197 A198 S228 Q252 Y254 T280 K349 H373 G374 R376 D409 G410 L431 G432 R433	Y135 S186 A187 T188 A189 S219 Q243 Y245 T271 K318 H342 G343 R345 D378 G379 L400 G401 R402
BS03	PPY	A	F143 L199 L286 H373	F134 L190 L277 H342

Gene Ontology

Molecular Function

• GO:0016491 oxidoreductase activity
• GO:0020037 heme binding

External links

• PDB: RCSB:1lco, PDBe:1lco, PDBj:1lco
• PDBsum: 1lco
• PubMed: 7632684
• UniProt: P00175|CYB2_YEAST L-lactate dehydrogenase (cytochrome) (Gene Name=CYB2)