Structure of PDB 1l8t Chain A

Receptor sequence
>1l8tA (length=263) Species: 1351 (Enterococcus faecalis) [Search protein sequence]
AKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKMTDSRYK
GTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEE
YEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDL
ADVDCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDG
KVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGIKPDW
EKIKYYILLDELF
3D structure
PDB1l8t Substrate promiscuity of an aminoglycoside antibiotic resistance enzyme via target mimicry.
ChainA
Resolution2.4 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) K44 D190 N195 D208
Catalytic site (residue number reindexed from 1) K43 D189 N194 D207
Enzyme Commision number 2.7.1.95: kanamycin kinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG A N195 D208 N194 D207
BS02 ADP A D22 G25 S27 Y42 K44 M90 E92 A93 S194 F197 I207 D208 D21 G24 S26 Y41 K43 M89 E91 A92 S193 F196 I206 D207
BS03 KAN A M26 E157 N158 E160 D190 R226 E230 D261 E262 F264 M25 E156 N157 E159 D189 R225 E229 D260 E261 F263
Gene Ontology
Molecular Function
GO:0005524 ATP binding
GO:0008910 kanamycin kinase activity
GO:0016301 kinase activity
GO:0016773 phosphotransferase activity, alcohol group as acceptor
Biological Process
GO:0016310 phosphorylation
GO:0046677 response to antibiotic

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:1l8t, PDBe:1l8t, PDBj:1l8t
PDBsum1l8t
PubMed12006485
UniProtP0A3Y5|KKA3_ENTFL Aminoglycoside 3'-phosphotransferase (Gene Name=aphA)

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