Structure of PDB 1kzn Chain A

Receptor sequence
>1kznA (length=186) Species: 562 (Escherichia coli) [Search protein sequence]
GLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCKEIIVTIH
ADNSVSVQDDGRGIPTGIGVSAAEVIMTVLHAHGVGVSVVNALSQKLELV
IQREGKIHRQIYEHGVPQAPLAVTGETEKTGTMVRFWPSLETFTNVTEFE
YEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHYE
3D structure
PDB1kzn DNA gyrase interaction with coumarin-based inhibitors: the role of the hydroxybenzoate isopentenyl moiety and the 5'-methyl group of the noviose.
ChainA
Resolution2.3 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 5.6.2.2: DNA topoisomerase (ATP-hydrolyzing).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 CBN A N46 E50 V71 D73 R76 G77 I78 P79 I90 R136 T165 N32 E36 V57 D59 R62 G63 I64 P65 I76 R103 T132 MOAD: Kd=1.2nM
PDBbind-CN: -logKd/Ki=8.92,Kd=1.2nM
BindingDB: IC50=73nM
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
GO:0005524 ATP binding
Biological Process
GO:0006265 DNA topological change

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Molecular Function
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Biological Process
External links
PDB RCSB:1kzn, PDBe:1kzn, PDBj:1kzn
PDBsum1kzn
PubMed12044152
UniProtP0AES6|GYRB_ECOLI DNA gyrase subunit B (Gene Name=gyrB)

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