Structure of PDB 1kms Chain A

Receptor sequence
>1kmsA (length=184) Species: 9606 (Homo sapiens) [Search protein sequence]
SLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIM
GKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQ
PELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEID
LEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND
3D structure
PDB1kms Atomic structures of human dihydrofolate reductase complexed with NADPH and two lipophilic antifolates at 1.09 a and 1.05 a resolution.
ChainA
Resolution1.09 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) L22 E30
Catalytic site (residue number reindexed from 1) L20 E28
Enzyme Commision number 1.5.1.3: dihydrofolate reductase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 LIH A I7 V8 E30 F31 F34 P61 V115 I5 V6 E28 F29 F32 P59 V113 BindingDB: IC50=0.98nM
BS02 NDP A V8 A9 I16 G17 G20 D21 L22 G53 K54 K55 T56 L75 S76 R77 E78 V115 G117 S118 V120 T146 V6 A7 I14 G15 G18 D19 L20 G51 K52 K53 T54 L73 S74 R75 E76 V113 G115 S116 V118 T144
Gene Ontology
Molecular Function
GO:0000900 mRNA regulatory element binding translation repressor activity
GO:0003723 RNA binding
GO:0003729 mRNA binding
GO:0004146 dihydrofolate reductase activity
GO:0005542 folic acid binding
GO:0016491 oxidoreductase activity
GO:0050661 NADP binding
GO:0070402 NADPH binding
GO:1990825 sequence-specific mRNA binding
Biological Process
GO:0006729 tetrahydrobiopterin biosynthetic process
GO:0006730 one-carbon metabolic process
GO:0017148 negative regulation of translation
GO:0031103 axon regeneration
GO:0031427 response to methotrexate
GO:0046452 dihydrofolate metabolic process
GO:0046653 tetrahydrofolate metabolic process
GO:0046654 tetrahydrofolate biosynthetic process
GO:0046655 folic acid metabolic process
GO:0051000 positive regulation of nitric-oxide synthase activity
GO:2000121 regulation of removal of superoxide radicals
Cellular Component
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1kms, PDBe:1kms, PDBj:1kms
PDBsum1kms
PubMed12096917
UniProtP00374|DYR_HUMAN Dihydrofolate reductase (Gene Name=DHFR)

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