Structure of PDB 1kh4 Chain A

Receptor sequence
>1kh4A (length=444) Species: 562 (Escherichia coli) [Search protein sequence]
VLENRAAQGNITAPGGARRLTGDQTAALRNSLSDKPAKNIILLIGDGMGD
SEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPDYVTDSAAS
ATAWSTGVKTYNGALGVDIHEKDHPTILEMAKAAGLATGNVSTAELQDAT
PAALVAHVTSRKCYGPSATSQKCPGNALEKGGKGSITEQLLNARADVTLG
GGAKTFAETATAGEWQGKTLREEAEARGYQLVSDAASLNSVTEANQQKPL
LGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDK
AIELLSKNEKGFFLQVEGASIDKQNHAANPCGQIGETVDLDEAVQRALEF
AKKEGNTLVIVTADHAHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNS
EEDSQEHTGSQLRIAAYGPHAANVVGLTDQTDLFYTMKAALGLK
3D structure
PDB1kh4 Artificial evolution of an enzyme active site: structural studies of three highly active mutants of Escherichia coli alkaline phosphatase.
ChainA
Resolution2.4 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D51 S102 D153 T155 R166 E322 D327 K328 H331 D369 H370 H412
Catalytic site (residue number reindexed from 1) D46 S97 D148 T150 R161 E317 D322 K323 H326 D364 H365 H407
Enzyme Commision number 3.1.3.1: alkaline phosphatase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ZN A D327 H331 H412 D322 H326 H407
BS02 ZN A D51 D369 H370 D46 D364 H365
BS03 MG A D51 T155 E322 D46 T150 E317
BS04 PO4 A S102 R166 D327 H412 S97 R161 D322 H407
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0004035 alkaline phosphatase activity
GO:0004721 phosphoprotein phosphatase activity
GO:0005515 protein binding
GO:0008270 zinc ion binding
GO:0016787 hydrolase activity
GO:0016791 phosphatase activity
GO:0030613 oxidoreductase activity, acting on phosphorus or arsenic in donors
GO:0033748 hydrogenase (acceptor) activity
GO:0046872 metal ion binding
Biological Process
GO:0006470 protein dephosphorylation
Cellular Component
GO:0030288 outer membrane-bounded periplasmic space
GO:0042597 periplasmic space

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Biological Process
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Cellular Component
External links
PDB RCSB:1kh4, PDBe:1kh4, PDBj:1kh4
PDBsum1kh4
PubMed11884134
UniProtP00634|PPB_ECOLI Alkaline phosphatase (Gene Name=phoA)

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