Structure of PDB 1kfs Chain A

Receptor sequence
>1kfsA (length=601) Species: 562 (Escherichia coli) [Search protein sequence]
MISYDNYVTILDEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSF
AIEPGVAAYIPVAHDYLDAPDQISRERALELLKPLLEDEKALKVGQNLKY
DRGILANYGIELRGIAFDTMLESYILNSVAGRHDMDSLAERWLKHKTITF
EEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQLHLKMWPDLQKHKGPL
NVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAELEKKAHEI
AGEEFNLSSTKQLQTILFEKQGIKPLKKTPSTSEEVLEELALDYPLPKVI
LEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQ
NIPVRNEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAF
AEGKDIHRATAAEVFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQL
NIPRKEAQKYMDLYFERYPGVLEYMERTRAQAKEQGYVETLDGRRLYLPD
IKSSNGARRAAAERAAINAPMQGTAADIIKRAMIAVDAWLQAEQPRVRMI
MQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPLLVEVGSGENWDQA
H
3D structure
PDB1kfs Structural principles for the inhibition of the 3'-5' exonuclease activity of Escherichia coli DNA polymerase I by phosphorothioates.
ChainA
Resolution2.1 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.7.7: DNA-directed DNA polymerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A D355 E357 T358 L361 Q419 N420 K422 Y423 R455 D457 F473 Y497 S658 H660 D32 E34 T35 L38 Q96 N97 K99 Y100 R132 D134 F150 Y174 S331 H333
BS02 ZN A D355 E357 D501 D32 E34 D178
BS03 ZN A H901 E905 H574 E578
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003887 DNA-directed DNA polymerase activity
GO:0008408 3'-5' exonuclease activity
Biological Process
GO:0006139 nucleobase-containing compound metabolic process
GO:0006260 DNA replication
GO:0006261 DNA-templated DNA replication

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Molecular Function
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Biological Process
External links
PDB RCSB:1kfs, PDBe:1kfs, PDBj:1kfs
PDBsum1kfs
PubMed9514742
UniProtP00582|DPO1_ECOLI DNA polymerase I (Gene Name=polA)

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