Structure of PDB 1kfq Chain A

Receptor sequence

>1kfqA (length=571) Species: 5888 (Paramecium tetraurelia)

QQVIPAPRVQVTQPYAGQKPGTSGLRKKVSEATQPNYLENFVQSIFNTLR
KDELKPKNVLFVGGDGRYFNRQAIFSIIRLAYANDISEVHVGQAGLMSTP
ASSHYIRKVNEEVGNCIGGIILTASHNPGGKEHGDFGIKFNVRTGAPAPE
DFTDQIYTHTTKIKEYLTVDYEFEKHINLDQIGVYKFEGTRLEKSHFEVK
VVDTVQDYTQLMQKLFDFDLLKGLFSNKDFSFRFDGMHGVAGPYAKHIFG
TLLGCSKESLLNCDPSEDFGGGHPDPNLTYAHDLVELLDIHKKKDVGTVP
QFGAACDGDADRNMILGRQFFVTPSDSLAVIAANANLIFKNGLLGAARSM
PTSGALDKVAAKNGIKLFETPTGWKFFGNLMDAGLINLCGEESFGTGSNH
IREKDGIWAVLAWLTILAHKNKNTDHFVTVEEIVTQYWQQFGRNYYSRYD
YEQVDSAGANKMMEHLKTKFQYFEQLKQGNKADIYDYVDPVDQSVSKNQG
VRFVFGDGSRIIFRLSGTGSVGATIRIYFEQFEQQQIQHETATALANIIK
LGLEISDIAQFTGRNEPTVIT

3D structure

• PDB: 1kfq Crystal structure analysis of the exocytosis-sensitive phosphoprotein, pp63/parafusin (phosphoglucomutase), from Paramecium reveals significant conformational variability.
• Chain: A
• Resolution: 2.4 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): S126 H127 D308 D310 D312 R313 K405
• Catalytic site (residue number reindexed from 1): S125 H126 D307 D309 D311 R312 K404
• Enzyme Commision number: 5.4.2.2: phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	A	S126 D308 D310 D312	S125 D307 D309 D311

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0004614 phosphoglucomutase activity
• GO:0016853 isomerase activity
• GO:0016868 intramolecular phosphotransferase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0005975 carbohydrate metabolic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:1kfq, PDBe:1kfq, PDBj:1kfq
• PDBsum: 1kfq
• PubMed: 11779235
• UniProt: P47244|PGM1_PARTE Phosphoglucomutase-1 (Gene Name=pp63-1)