Structure of PDB 1kcw Chain A

Receptor sequence

>1kcwA (length=1017) Species: 9606 (Homo sapiens)

KEKHYYIGIIETTWDYASDHGEKKLISVDTEHSNIYLQNGPDRIGRLYKK
ALYLQYTDETFRTTIEKPVWLGFLGPIIKAETGDKVYVHLKNLASRPYTF
HSHGITYYKEHEGAIYPDNTTDFQRADDKVYPGEQYTYMLLATEEQSPGE
GDGNCVTRIYHSHIDAPKDIASGLIGPLIICKKDSLDKEKEKHIDREFVV
MFSVVDENFSWYLEDNIKTYCSEPEKVDKDNEDFQESNRMYSVNGYTFGS
LPGLSMCAEDRVKWYLFGMGNEVDVHAAFFHGQALTNKNYRIDTINLFPA
TLFDAYMVAQNPGEWMLSCQNLNHLKAGLQAFFQVQECIRGKHVRHYYIA
AEEIIWNYAPSGIDIFTKENLTAPGSDSAVFFEQGTTRIGGSYKKLVYRE
YTDASFTNRKERGPEEEHLGILGPVIWAEVGDTIRVTFHNKGAYPLSIEP
IGVRFNKNNEGTYYSPVPPSASHVAPTETFTYEWTVPKEVGPTNADPVCL
AKMYYSAVDPTKDIFTGLIGPMKICKKGSLHANGRQKDVDKEFYLFPTVF
DENESLLLEDNIRMFTTAPDQVDKEDEDFQESNKMHSMNGFMYGNQPGLT
MCKGDSVVWYLFSAGNEADVHGIYFSGNTYLWRGERRDTANLFPQTSLTL
HMWPDTEGTFNVECLTTDHYTGGMKQKYTVNQCRRQSEDSTFYLGERTYY
IAAVEVEWDYSPQREWEKELHHLQEQNVSNAFLDKGEFYIGSKYKKVVYR
QYTDSTFRVPVERKAEEEHLGILGPQLHADVGDKVKIIFKNMATRPYSIH
AHGVQTESSTVTPTLPGETLTYVWKIPERSGAGTEDSACIPWAYYSTVDQ
VKDLYSGLIGPLIVCRRPRRKLEFALLFLVFDENESWYLDDNIKTYSDHP
EKVNKDDEEFIESNKMHAINGRMFGNLQGLTMHVGDEVNWYLMGMGNEID
LHTVHFHGHSFQYKHRGVYSSDVFDIFPGTYQTLEMFPRTPGIWLLHCHV
TDHIHAGMETTYTVLQN

3D structure

• PDB: 1kcw The X-ray structure of human serum ceruloplasmin at 3.1 angstrom: Nature of the copper centres.
• Chain: A
• Resolution: 3.0 Å

Enzymatic activity

• Enzyme Commision number: 1.11.1.27: glutathione-dependent peroxiredoxin.
  1.11.1.9: glutathione peroxidase.
  1.16.3.1: ferroxidase.
  1.16.3.4: cuproxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CU	A	H276 C319 H324	H276 C319 H324
BS02	CU	A	H163 H980 H1020	H163 H957 H997
BS03	CU	A	H103 H161 H1022	H103 H161 H999
BS04	CU	A	H101 H103 H978 H980	H101 H103 H955 H957
BS05	CU	A	H637 C680 H685	H621 C664 H669
BS06	CU	A	H602 D684	H586 D668
BS07	CU	A	H975 C1021 H1026	H952 C998 H1003
BS08	CU	A	H940 D1025	H917 D1002
BS09	O	A	H161 H163 H980 H1020	H161 H163 H957 H997
BS10	O	A	H101 H103 H978	H101 H103 H955

Gene Ontology

Molecular Function

• GO:0004322 ferroxidase activity
• GO:0004602 glutathione peroxidase activity
• GO:0005507 copper ion binding
• GO:0016491 oxidoreductase activity
• GO:0016724 oxidoreductase activity, acting on metal ions, oxygen as acceptor
• GO:0046872 metal ion binding
• GO:0047066 phospholipid-hydroperoxide glutathione peroxidase activity
• GO:0051087 protein-folding chaperone binding

Biological Process

• GO:0006826 iron ion transport
• GO:0006878 intracellular copper ion homeostasis
• GO:0006879 intracellular iron ion homeostasis
• GO:0098869 cellular oxidant detoxification

Cellular Component

• GO:0005576 extracellular region
• GO:0005615 extracellular space
• GO:0005765 lysosomal membrane
• GO:0005788 endoplasmic reticulum lumen
• GO:0005886 plasma membrane
• GO:0070062 extracellular exosome
• GO:0072562 blood microparticle

External links

• PDB: RCSB:1kcw, PDBe:1kcw, PDBj:1kcw
• PDBsum: 1kcw
• UniProt: P00450|CERU_HUMAN Ceruloplasmin (Gene Name=CP)