Structure of PDB 1kbi Chain A

Receptor sequence

>1kbiA (length=504) Species: 4932 (Saccharomyces cerevisiae)

EPKLDMNKQKISPAEVAKHNKPDDCWVVINGYVYDLTRFLPNHPGGQDVI
KFNAGKDVTAIFEPLHAPNVIDKYIAPEKKLGPLQGSMPPELVCPPYAPG
ETKEDIARKEQLKSLLPPLDNIINLYDFEYLASQTLTKQAWAYYSSGAND
EVTHRENHNAYHRIFFKPKILVDVRKVDISTDMLGSHVDVPFYVSATALC
KLGNPLEGEKDVARGCGQGVTKVPQMISTLASCSPEEIIEAAPSDKQIQW
YQLYVNSDRKITDDLVKNVEKLGVKALFVTVDAPSLGQREKDMKLKFSNT
KKTNVEESQGASRALSKFIDPSLTWKDIEELKKKTKLPIVIKGVQRTEDV
IKAAEIGVSGVVLSNHGGRQLDFSRAPIEVLAETMPILEQRNLKDKLEVF
VDGGVRRGTDVLKALCLGAKGVGLGRPFLYANSCYGRNGVEKAIEILRDE
IEMSMRLLGVTSIAELKPDLLDLSTLKARTVGVPNDVLYNEVYEGPTLTE
FEDA

3D structure

• PDB: 1kbi Crystallographic study of the recombinant flavin-binding domain of Baker's yeast flavocytochrome b(2): comparison with the intact wild-type enzyme.
• Chain: A
• Resolution: 2.3 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): S228 Y254 T280 D282 K349 H373
• Catalytic site (residue number reindexed from 1): S228 Y254 T280 D282 K342 H366
• Enzyme Commision number: 1.1.2.3: L-lactate dehydrogenase (cytochrome).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEM	A	F39 H43 P44 G45 V49 I50 V58 I61 F62 H66 V70 Y97 Q139 Y143 A198 L230 K296	F39 H43 P44 G45 V49 I50 V58 I61 F62 H66 V70 Y97 Q139 Y143 A198 L230 K296
BS02	FMN	A	Y143 Y144 S195 A196 T197 A198 S228 Q252 Y254 T280 K349 H373 G374 R376 D409 G410 R413 G432 R433	Y143 Y144 S195 A196 T197 A198 S228 Q252 Y254 T280 K342 H366 G367 R369 D402 G403 R406 G425 R426

Gene Ontology

Molecular Function

• GO:0016491 oxidoreductase activity
• GO:0020037 heme binding

External links

• PDB: RCSB:1kbi, PDBe:1kbi, PDBj:1kbi
• PDBsum: 1kbi
• PubMed: 11914072
• UniProt: P00175|CYB2_YEAST L-lactate dehydrogenase (cytochrome) (Gene Name=CYB2)