Structure of PDB 1k98 Chain A

Receptor sequence

>1k98A (length=577) Species: 562 (Escherichia coli)

QAEWRSWEVNKRLEYSLVKGITEFIEQDTEEARQQATRPIEVIEGPLMDG
MNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASKEQGKTNGKMV
IATVKGDVGDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNADL
IGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNY
SGPTVYVQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPR
TPPVTLEAARDNDFAFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPF
FMTWSLAGKYPRILEDEVVGVEAQRLFKDANDMLDKLSAEKTLNPRGVVG
LFPANRVGDDIEIYRDETRTHVINVSHHLRQQTEKTGFANYCLADFVAPK
LSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMVKALADRLAEAFA
EYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHTEKAT
IWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQV
EDYARRKGMSVTEVERWLAPNLGYDAD

3D structure

• PDB: 1k98 Domain alternation switches B(12)-dependent methionine synthase to the activation conformation.
• Chain: A
• Resolution: 3.75 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D757 G759 S810
• Catalytic site (residue number reindexed from 1): D107 G109 S160
• Enzyme Commision number: 2.1.1.13: methionine synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	B12	A	G759 G762 V766 G802 L803 L806 I807 T808 G833 G834 A835 V857 N859 A860 D1093 P1137 G1138 Y1139 H1145 A1170 M1171 G1174 S1176 V1177 S1178	G109 G112 V116 G152 L153 L156 I157 T158 G183 G184 A185 V207 N209 A210 D443 P487 G488 Y489 H495 A520 M521 G524 S526 V527 S528

Gene Ontology

Molecular Function

• GO:0008705 methionine synthase activity
• GO:0031419 cobalamin binding
• GO:0046872 metal ion binding

Biological Process

• GO:0009086 methionine biosynthetic process

External links

• PDB: RCSB:1k98, PDBe:1k98, PDBj:1k98
• PDBsum: 1k98
• PubMed: 11731805
• UniProt: P13009|METH_ECOLI Methionine synthase (Gene Name=metH)