Structure of PDB 1jfh Chain A

Receptor sequence
>1jfhA (length=496) Species: 9823 (Sus scrofa) [Search protein sequence]
QYAPQTQSGRTSIVHLFEWRWVDIALECERYLGPKGFGGVQVSPPNENVV
VTNPSRPWWERYQPVSYKLCTRSGNENEFRDMVTRCNNVGVRIYVDAVIN
HMCGSGAAAGTGTTCGSYCNPGSREFPAVPYSAWDFNDGKCKTASGGIES
YNDPYQVRDCQLVGLLDLALEKDYVRSMIADYLNKLIDIGVAGFRIDASK
HMWPGDIKAVLDKLHNLNTNWFPAGSRPFIFQEVIDLGGEAIKSSEYFGN
GRVTEFKYGAKLGTVVRKWSGEKMSYLKNWGEGWGFMPSDRALVFVDNHD
NQRGHGAGGSSILTFWDARLYKIAVGFMLAHPYGFTRVMSSYRWARNFVN
GEDVNDWIGPPNNNGVIKEVTINADTTCGNDWVCEHRWREIRNMVWFRNV
VDGEPFANWWDNGSNQVAFGRGNRGFIVFNNDDWQLSSTLQTGLPGGTYC
DVISGDKVGNSCTGIKVYVSSDGTAQFSISNSAEDPFIAIHAESKL
3D structure
PDB1jfh Structure of a pancreatic alpha-amylase bound to a substrate analogue at 2.03 A resolution.
ChainA
Resolution2.03 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D197 S226 D300
Catalytic site (residue number reindexed from 1) D197 S226 D300
Enzyme Commision number 3.2.1.1: alpha-amylase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MA3 A W59 Q63 H305 W59 Q63 H305 PDBbind-CN: -logKd/Ki=2.05,Ki=9mM
BS02 MA2 A W59 V163 W59 V163 PDBbind-CN: -logKd/Ki=2.05,Ki=9mM
BS03 MA1 A K200 E240 G306 A307 K200 E240 G306 A307
BS04 MA1 A A318 K322 W388 A318 K322 W388
BS05 GLC A R387 R389 E390 R387 R389 E390
BS06 CA A N100 R158 D167 H201 N100 R158 D167 H201
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0004556 alpha-amylase activity
GO:0005509 calcium ion binding
GO:0016160 amylase activity
GO:0016798 hydrolase activity, acting on glycosyl bonds
GO:0031404 chloride ion binding
GO:0043169 cation binding
GO:0046872 metal ion binding
Biological Process
GO:0005975 carbohydrate metabolic process
GO:0016052 carbohydrate catabolic process
Cellular Component
GO:0005576 extracellular region
GO:0005615 extracellular space

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1jfh, PDBe:1jfh, PDBj:1jfh
PDBsum1jfh
PubMed9385631
UniProtP00690|AMYP_PIG Pancreatic alpha-amylase (Gene Name=AMY2)

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