Structure of PDB 1j4x Chain A

Receptor sequence
>1j4xA (length=178) Species: 9606 (Homo sapiens) [Search protein sequence]
SVQDLNDLLSDGSGCYSLPSQPCNEVTPRIYVGNASVAQDIPKLQKLGIT
HVLNAAEGRSFMHVNTNANFYKDSGITYLGIKANDTQEFNLSAYFERAAD
FIDQALAQKNGRVLVHSREGYSRSPTLVIAYLMMRQKMDVKSALSIVRQN
REIGPNDGFLAQLCQLNDRLAKEGKLKP
3D structure
PDB1j4x Structural basis for the recognition of a bisphosphorylated MAP kinase peptide by human VHR protein Phosphatase.
ChainA
Resolution2.75 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.3.16: protein-serine/threonine phosphatase.
3.1.3.48: protein-tyrosine-phosphatase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 peptide A Y23 L25 P26 S27 Q28 D92 Q94 S124 R125 E126 Y128 S129 R130 G161 P162 N163 D164 Y16 L18 P19 S20 Q21 D85 Q87 S117 R118 E119 Y121 S122 R123 G154 P155 N156 D157
Gene Ontology
Molecular Function
GO:0004721 phosphoprotein phosphatase activity
GO:0004722 protein serine/threonine phosphatase activity
GO:0004725 protein tyrosine phosphatase activity
GO:0005515 protein binding
GO:0008092 cytoskeletal protein binding
GO:0008138 protein tyrosine/serine/threonine phosphatase activity
GO:0016791 phosphatase activity
GO:0017018 myosin phosphatase activity
GO:0019901 protein kinase binding
GO:0030971 receptor tyrosine kinase binding
GO:0033549 MAP kinase phosphatase activity
GO:1990782 protein tyrosine kinase binding
Biological Process
GO:0006470 protein dephosphorylation
GO:0016311 dephosphorylation
GO:0030336 negative regulation of cell migration
GO:0035335 peptidyl-tyrosine dephosphorylation
GO:0042059 negative regulation of epidermal growth factor receptor signaling pathway
GO:0043409 negative regulation of MAPK cascade
GO:0045931 positive regulation of mitotic cell cycle
GO:0046329 negative regulation of JNK cascade
GO:0050860 negative regulation of T cell receptor signaling pathway
GO:0050868 negative regulation of T cell activation
GO:0050922 negative regulation of chemotaxis
GO:0051893 regulation of focal adhesion assembly
GO:0070373 negative regulation of ERK1 and ERK2 cascade
GO:0071364 cellular response to epidermal growth factor stimulus
GO:0120183 positive regulation of focal adhesion disassembly
GO:1990264 peptidyl-tyrosine dephosphorylation involved in inactivation of protein kinase activity
Cellular Component
GO:0001772 immunological synapse
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005856 cytoskeleton
GO:0031514 motile cilium

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1j4x, PDBe:1j4x, PDBj:1j4x
PDBsum1j4x
PubMed11863439
UniProtP51452|DUS3_HUMAN Dual specificity protein phosphatase 3 (Gene Name=DUSP3)

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