Structure of PDB 1j0j Chain A

Receptor sequence
>1j0jA (length=588) Species: 1422 (Geobacillus stearothermophilus) [Search protein sequence]
MRKEAIYHRPADNFAYAYDSETLHLRLRTKKDDIDRVELLHGDPYDWQNG
AWQFQMMPMRKTGSDELFDYWFAEVKPPYRRLRYGFVLYSGEEKLVYTEK
GFYFEVPTDDTAYYFCFPFLHRVDLFEAPDWVKDTVWYQIFPERFANGNP
SISPEGSRPWGSEDPTPTSFFGGDLQGIIDHLDYLVDLGITGIYLTPIFR
SPSNHKYDTADYFEVDPHFGDKETLKTLIDRCHEKGIRVMLDAVFNHCGY
EFAPFQDVWKNGESSKYKDWFHIHEFPLQTEPRPNYDTFAFVPQMPKLNT
ANPEVKRYLLDVATYWIREFDIDGWRLDVANEIDHEFWREFRQEVKALKP
DVYILGQIWHDAMPWLRGDQFDAVMNYPFTDGVLRFFAKEEISARQFANQ
MMHVLHSYPNNVNEAAFNLLGSHDTSRILTVCGGDIRKVKLLFLFQLTFT
GSPCIYYGDEIGMTGGNDPECRKCMVWDPMQQNKELHQHVKQLIALRKQY
RSLRRGEISFLHADDEMNYLIYKKTDGDETVLVIINRSDQKADIPIPLDA
RGTWLVNLLTGERFAAEAETLCTSLPPYGFVLYAIEHW
3D structure
PDB1j0j Three-dimensional structure and substrate binding of Bacillus stearothermophilus neopullulanase
ChainA
Resolution2.8 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D242 R326 D328 Q357 H423 D424
Catalytic site (residue number reindexed from 1) D242 R326 D328 Q357 H423 D424
Enzyme Commision number 3.2.1.135: neopullulanase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 GLC A F289 V329 Q357 F289 V329 Q357
BS02 GLC A Y207 H247 F289 D328 Q357 H423 D424 Y207 H247 F289 D328 Q357 H423 D424
BS03 GLC A Y207 M295 D468 R472 Y207 M295 D468 R472
BS04 GLC A R83 T111 R83 T111
Gene Ontology
Molecular Function
GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
GO:0005509 calcium ion binding
GO:0005536 D-glucose binding
GO:0016798 hydrolase activity, acting on glycosyl bonds
GO:0031216 neopullulanase activity
GO:0042802 identical protein binding
GO:0042803 protein homodimerization activity
GO:0046872 metal ion binding
Biological Process
GO:0005975 carbohydrate metabolic process

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Molecular Function
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Biological Process
External links
PDB RCSB:1j0j, PDBe:1j0j, PDBj:1j0j
PDBsum1j0j
PubMed12547200
UniProtP38940|NEPU_GEOSE Neopullulanase (Gene Name=nplT)

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