Structure of PDB 1iyk Chain A

Receptor sequence

>1iykA (length=392) Species: 5476 (Candida albicans)

EGPIDKLKTPEDVPNDPLPLISDFEWSTLDIDDNLQLDELYKLLYDNYVE
DIDATFRFKYSHEFFQWALKPPGWRKDWHVGVRVKSTGKLVAFIAATPVT
FKLNKSNKVIDSVEINFLCIHKKLRNKRLAPVLIKEITRRVNKQNIWQAL
YTGGSILPTPLTTCRYQHRPINWSKLHDVGFSHLPPNQTKSSMVASYTLP
NNPKLKGLRPMTGKDVSTVLSLLYKYQERFDIVQLFTEEEFKHWMLGHDE
NSDSNVVKSYVVEDENGIITDYFSYYLLPFTVLDNAQHDELGIAYLFYYA
SDSFEKPNYKKRLNELITDALITSKKFGVDVFNCLTCQDNTYFLKDCKFG
SGDGFLNYYLFNYRTFPMDGGIDKKTKEVVEDQTSGIGVVLL

3D structure

• PDB: 1iyk Crystal Structures of Candida albicans N-Myristoyltransferase with Two Distinct Inhibitors
• Chain: A
• Resolution: 2.3 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): N175 F176 L177 T211 L451
• Catalytic site (residue number reindexed from 1): N116 F117 L118 T152 L392
• Enzyme Commision number: 2.3.1.97: glycylpeptide N-tetradecanoyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MYA	A	Y107 V108 I174 L177 I179 R184 N185 R187 A189 T197 W206 Q207 A208 Y210 T211 L216	Y48 V49 I115 L118 I120 R125 N126 R128 A130 T138 W147 Q148 A149 Y151 T152 L157
BS02	MIM	A	V108 D110 D112 F117 T211 Y225 H227 P229 F240 G409 G411 D412 G413 L451	V49 D51 D53 F58 T152 Y166 H168 P170 F181 G350 G352 D353 G354 L392	MOAD: Ki=0.25uM BindingDB: Ki=31nM

Gene Ontology

Molecular Function

• GO:0004379 glycylpeptide N-tetradecanoyltransferase activity

Biological Process

• GO:0006499 N-terminal protein myristoylation

External links

• PDB: RCSB:1iyk, PDBe:1iyk, PDBj:1iyk
• PDBsum: 1iyk
• PubMed: 12401496
• UniProt: P30418|NMT_CANAL Glycylpeptide N-tetradecanoyltransferase (Gene Name=NMT1)