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PDB

1ix3 Crystal Structures of Ferrous and CO-, CN(-)-, and NO-Bound Forms of Rat Heme Oxygenase-1 (HO-1) in Complex with Heme: Structural Implications for Discrimination between CO and O(2) in HO-1.

Chain

Resolution

2.0 Å

3D
structure

Catalytic site residues are labeled in the structure
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Site
#

Ligand

Ligand
chain

Binding residues on receptor
(original residue number in PDB)

Binding residues on receptor
(residue number reindexed from 1)

Binding affinity

BS01

HEM

K18 H25 E29 M34 Q38 Y134 T135 R136 G139 S142 G143 K179 R183 F207 N210

K8 H15 E19 M24 Q28 Y124 T125 R126 G129 S132 G133 K169 R173 F197 N200

Molecular Function

GO:0004392

heme oxygenase (decyclizing) activity

GO:0004630

phospholipase D activity

GO:0005198

structural molecule activity

GO:0005515

protein binding

GO:0016491

oxidoreductase activity

GO:0019899

enzyme binding

GO:0020037

heme binding

GO:0042802

identical protein binding

GO:0042803

protein homodimerization activity

GO:0046872

metal ion binding

GO:0052869

arachidonate omega-hydroxylase activity

	Biological Process
GO:0001525	angiogenesis
GO:0001666	response to hypoxia
GO:0002246	wound healing involved in inflammatory response
GO:0006357	regulation of transcription by RNA polymerase II
GO:0006644	phospholipid metabolic process
GO:0006788	heme oxidation
GO:0006879	intracellular iron ion homeostasis
GO:0006915	apoptotic process
GO:0006979	response to oxidative stress
GO:0007264	small GTPase-mediated signal transduction
GO:0008217	regulation of blood pressure
GO:0008285	negative regulation of cell population proliferation
GO:0008630	intrinsic apoptotic signaling pathway in response to DNA damage
GO:0009410	response to xenobiotic stimulus
GO:0010656	negative regulation of muscle cell apoptotic process
GO:0016236	macroautophagy
GO:0016239	positive regulation of macroautophagy
GO:0016242	negative regulation of macroautophagy
GO:0031670	cellular response to nutrient
GO:0032764	negative regulation of mast cell cytokine production
GO:0034101	erythrocyte homeostasis
GO:0034605	cellular response to heat
GO:0035094	response to nicotine
GO:0035556	intracellular signal transduction
GO:0042167	heme catabolic process
GO:0042168	heme metabolic process
GO:0042542	response to hydrogen peroxide
GO:0043305	negative regulation of mast cell degranulation
GO:0043524	negative regulation of neuron apoptotic process
GO:0043627	response to estrogen
GO:0045766	positive regulation of angiogenesis
GO:0048661	positive regulation of smooth muscle cell proliferation
GO:0048662	negative regulation of smooth muscle cell proliferation
GO:0060586	multicellular organismal-level iron ion homeostasis
GO:0071243	cellular response to arsenic-containing substance
GO:0071276	cellular response to cadmium ion
GO:0072719	cellular response to cisplatin
GO:0090050	positive regulation of cell migration involved in sprouting angiogenesis
GO:0097421	liver regeneration
GO:0110076	negative regulation of ferroptosis
GO:1902042	negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
GO:1903589	positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis
GO:1904019	epithelial cell apoptotic process
GO:1904036	negative regulation of epithelial cell apoptotic process
GO:1904037	positive regulation of epithelial cell apoptotic process
GO:1904550	response to arachidonate
GO:1904681	response to 3-methylcholanthrene
GO:1904706	negative regulation of vascular associated smooth muscle cell proliferation

Biological Process

GO:0001525

angiogenesis

GO:0001666

response to hypoxia

GO:0002246

wound healing involved in inflammatory response

GO:0006357

regulation of transcription by RNA polymerase II

GO:0006644

phospholipid metabolic process

GO:0006788

heme oxidation

GO:0006879

intracellular iron ion homeostasis

GO:0006915

apoptotic process

GO:0006979

response to oxidative stress

GO:0007264

small GTPase-mediated signal transduction

GO:0008217

regulation of blood pressure

GO:0008285

negative regulation of cell population proliferation

GO:0008630

intrinsic apoptotic signaling pathway in response to DNA damage

GO:0009410

response to xenobiotic stimulus

GO:0010656

negative regulation of muscle cell apoptotic process

GO:0016236

macroautophagy

GO:0016239

positive regulation of macroautophagy

GO:0016242

negative regulation of macroautophagy

GO:0031670

cellular response to nutrient

GO:0032764

negative regulation of mast cell cytokine production

GO:0034101

erythrocyte homeostasis

GO:0034605

cellular response to heat

GO:0035094

response to nicotine

GO:0035556

intracellular signal transduction

GO:0042167

heme catabolic process

GO:0042168

heme metabolic process

GO:0042542

response to hydrogen peroxide

GO:0043305

negative regulation of mast cell degranulation

GO:0043524

negative regulation of neuron apoptotic process

GO:0043627

response to estrogen

GO:0045766

positive regulation of angiogenesis

GO:0048661

positive regulation of smooth muscle cell proliferation

GO:0048662

negative regulation of smooth muscle cell proliferation

GO:0060586

multicellular organismal-level iron ion homeostasis

GO:0071243

cellular response to arsenic-containing substance

GO:0071276

cellular response to cadmium ion

GO:0072719

cellular response to cisplatin

GO:0090050

positive regulation of cell migration involved in sprouting angiogenesis

GO:0097421

liver regeneration

GO:0110076

negative regulation of ferroptosis

GO:1902042

negative regulation of extrinsic apoptotic signaling pathway via death domain receptors

GO:1903589

positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis

GO:1904019

epithelial cell apoptotic process

GO:1904036

negative regulation of epithelial cell apoptotic process

GO:1904037

positive regulation of epithelial cell apoptotic process

GO:1904550

response to arachidonate

GO:1904681

response to 3-methylcholanthrene

GO:1904706

negative regulation of vascular associated smooth muscle cell proliferation

	Cellular Component
GO:0005634	nucleus
GO:0005783	endoplasmic reticulum
GO:0005789	endoplasmic reticulum membrane
GO:0005829	cytosol
GO:0005901	caveola
GO:0016020	membrane
GO:0048471	perinuclear region of cytoplasm

Cellular Component

GO:0005634

nucleus

GO:0005783

endoplasmic reticulum

GO:0005789

endoplasmic reticulum membrane

cytosol

caveola

membrane

perinuclear region of cytoplasm

PDB	RCSB:1ix3, PDBe:1ix3, PDBj:1ix3
PDBsum	1ix3
PubMed	12924938
UniProt	P06762\|HMOX1_RAT Heme oxygenase 1 (Gene Name=Hmox1)

Structure of PDB 1ix3 Chain A